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SP140 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3201205

This is a rabbit polyclonal antibody against SP140. It was validated on Western Blot and immunohistochemistryTarget Description: SP140 is the nuclear body protein found specifically in all NP cells. HIV-1 infection induced its partial dispersal from nuclear… More details

$440 / 0.1 mL +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SP140, Polyclonal Antibody
Full Product Name
SP140 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
LYSP100; LYSP100-A; LYSP100-B
Immunogen Sequence
Synthetic peptide located within the following region: PEPIFRFFRE NKVEIASAIT RPFPFLMGLR DRSFISEQMY EHFQEAFRNL
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Dog, Horse, Pig
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Protein A purified
Concentration
1.0 mg/ml (lot specific)
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
SP140 peptide (MBS3226229) is used for blocking the activity of SP140 antibody (MBS3201205)
Molecular Weight
46kDa
OMIM
608602
3D Structure
ModBase 3D Structure for Q13342
Homology
Dog: 86%; Horse: 86%; Human: 100%; Mouse: 77%; Pig: 86%; Rat: 77%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human SP140
Protein Size (# AA)
412 amino acids
Protein Interactions
UBC; Sertad1; SIRT2;
Protein Name
Nuclear body protein SP140
Blocking Peptide
For anti-SP140 (MBS3201205) antibody is Catalog # MBS3226229
Replacement Item
This antibody may replace item sc-109035 from Santa Cruz Biotechnology.
Applications Tested/Suitable for anti-SP140 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-SP140 antibody
IHC Information: Raji Cell Lysate

Immunohistochemistry (IHC) of anti-SP140 antibody

Rabbit Anti-SP 140 Antibody
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Rabbit Anti-SP140 Polyclonal Antibody – Cat# MBS3201205

Western Blot (WB) of anti-SP140 antibody

Host: Rabbit
Target Name: SP140
Sample Type: Human Lung Tumor
Antibody Dilution: 0.5 ug/ml
Rabbit Anti-SP140 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3201205

Western Blot (WB) of anti-SP140 antibody

WB Suggested Anti-SP140 Antibody Titration: 5.0ug/ml
Positive Control: Raji cell lysateSP140 is supported by BioGPS gene expression data to be expressed in Raji
Rabbit Anti-SP140 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3201205

Other Notes

Small volumes of anti-SP140 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SP140 purchase

MBS3201205 is an antibody targeting SP140. Also known as SP140 antibody - N-terminal region. Reactive in Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Dog, Horse, Pig. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SP140. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nuclear body protein SP140 isoform 2
NCBI Official Synonym Full Names
SP140 nuclear body protein
NCBI Official Symbol
SP140
NCBI Official Synonym Symbols
LYSP100; LYSP100-A; LYSP100-B
UniProt Protein Name
Nuclear body protein SP140
UniProt Synonym Protein Names
Lymphoid-restricted homolog of Sp100; Nuclear autoantigen Sp-140; Speckled 140 kDa
Protein Family
UniProt Gene Name
SP140
UniProt Entry Name
SP140_HUMAN
NCBI Protein Information
nuclear body protein SP140

NCBI Summary for SP140

This gene encodes a member of the SP100 family of proteins, which are share common domains including an N-terminal homogeneously staining region domain followed by a SP100/autoimmune regulator/NucP41/P75/deformed epidermal autoregulatory factor domain, a plant homeobox zinc finger, and a bromodomain. The encoded protein is interferon-inducible and is expressed at high levels in the nuclei of leukocytes. Variants of this gene have been associated with multiple sclerosis, Crohn's disease, and chronic lymphocytic leukemia. Alternative splicing results in multiple variants. [provided by RefSeq, Aug 2016]
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UniProt Comments for SP140

SP140: Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; PML body; cytoplasm; nuclear envelope; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; chromatin binding; transcription factor activity

Biological Process: chromatin remodeling; regulation of transcription, DNA-dependent; defense response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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