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PCCA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3201311

This is a rabbit polyclonal antibody against PCCA. It was validated on Western Blot using a cell lysate as a positive control.Target Description: PCCA is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA is the… More details

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Product Overview

Online Datasheet in PDF
Product Name
PCCA, Polyclonal Antibody
Full Product Name
PCCA antibody - N-terminal region
Product Gene Name
anti-PCCA antibody
Immunogen Sequence
Synthetic peptide located within the following region: LYYSRQCLMV SRNLGSVGYD PNEKTFDKIL VANRGEIACR VIRTCKKMGI
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
PCCA peptide (MBS3226334) is used for blocking the activity of PCCA antibody (MBS3201311)
Molecular Weight
75kDa
OMIM
232000
3D Structure
ModBase 3D Structure for Q8WXQ7
Homology
Cow: 86%; Dog: 86%; Horse: 83%; Human: 100%; Mouse: 77%; Pig: 86%; Rabbit: 86%; Rat: 86%; Zebrafish: 75%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human PCCA

Product Categories/Family for anti-PCCA antibody

Polyclonal; Drugs and Drug Metabolism; Disease Related; DNA/RNA/Protein Interactions
Applications Tested/Suitable for anti-PCCA antibody
Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry (IHC) of anti-PCCA antibody

Rabbit Anti-PCCA Antibody
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Rabbit Anti-PCCA Polyclonal Antibody – Cat# MBS3201311

Western Blot (WB) of anti-PCCA antibody

WB Suggested Anti-PCCA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T
Rabbit Anti-PCCA Polyclonal Antibody, Western Blot (WB) – Cat# MBS3201311

Other Notes

Small volumes of anti-PCCA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCCA purchase

MBS3201311 is an antibody targeting PCCA. Also known as PCCA antibody - N-terminal region. Reactive in Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PCCA. It may not necessarily be applicable to this product.

NCBI GI #
65506442
NCBI GeneID
5095
NCBI Accession #
NP_000273
UniProt Primary Accession #
Q8WXQ7
UniProt Related Accession #
P05165
GenBank Nucleotide #
NM_000282
NCBI Official Full Name
propionyl-CoA carboxylase alpha chain, mitochondrial isoform a
NCBI Official Synonym Full Names
propionyl-CoA carboxylase subunit alpha
NCBI Official Symbol
PCCA
UniProt Protein Name
Propionyl-CoA carboxylase alpha chain, mitochondrial
UniProt Synonym Protein Names
Propanoyl-CoA:carbon dioxide ligase subunit alpha
UniProt Gene Name
PCCA
UniProt Entry Name
PCCA_HUMAN
NCBI Protein Information
propionyl-CoA carboxylase alpha chain, mitochondrial

NCBI Summary for PCCA

The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]
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UniProt Comments for PCCA

PCCA: Defects in PCCA are the cause of propionic acidemia type I (PA-1). PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 6.4.1.3; Ligase; Carbohydrate Metabolism - propanoate

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: mitochondrial matrix; cytosol

Molecular Function: enzyme binding; metal ion binding; propionyl-CoA carboxylase activity; biotin carboxylase activity; ATP binding; biotin binding

Biological Process: fatty acid beta-oxidation; vitamin metabolic process; short-chain fatty acid catabolic process; cellular lipid metabolic process; water-soluble vitamin metabolic process; biotin metabolic process

Disease: Propionic Acidemia
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Research Articles on PCCA

1. The data indicate that amino acid/nucleotide metabolism-related genes OGDH, PPAT and PCCA acquire somatic mutations in microsatellite instability-high gastric cancers and colorectal cancers and that mutational intratumoral heterogeneity may occur in at least some of these tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PCCA antibody

 Reference Product  PubMed Publications
 PCCB antibody  >53 publications with PCCA and PCCB
 ACAA2 antibody  >1 publications with PCCA and ACAA2
 MCEE antibody  >1 publications with PCCA and MCEE

Pathways associated with anti-PCCA antibody

 Products by Pathway  Pathway Diagram
 2-oxobutanoate Degradation Pathway antibodies  2-oxobutanoate Degradation Pathway Diagram
 2-oxobutanoate Degradation I Pathway antibodies  2-oxobutanoate Degradation I Pathway Diagram
 Biotin Transport And Metabolism Pathway antibodies  Biotin Transport And Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies  Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram

Diseases associated with anti-PCCA antibody

 Disease Name  Pubmed Publications
 Propionic Acidemia Antibodies  >53 publications with PCCA and Propionic Acidemia
 Carcinoma Antibodies  >7 publications with PCCA and Carcinoma
 Adenocarcinoma Antibodies  >7 publications with PCCA and Adenocarcinoma
 Fatty Liver Antibodies  >6 publications with PCCA and Fatty Liver
 Disease Models, Animal Antibodies  >6 publications with PCCA and Disease Models, Animal
 Liver Diseases Antibodies  >4 publications with PCCA and Liver Diseases
 Liver Neoplasms Antibodies  >4 publications with PCCA and Liver Neoplasms
 Atrophy Antibodies  >4 publications with PCCA and Atrophy
 Seizures Antibodies  >3 publications with PCCA and Seizures
 Heart Diseases Antibodies  >3 publications with PCCA and Heart Diseases

Organs/Tissues associated with anti-PCCA antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >43 publications with PCCA and Blood
 Liver Antibodies  >21 publications with PCCA and Liver
 Vascular Antibodies  >7 publications with PCCA and Vascular
 Skin Antibodies  >6 publications with PCCA and Skin
 Heart Antibodies  >5 publications with PCCA and Heart
 Muscle Antibodies  >5 publications with PCCA and Muscle
 Brain Antibodies  >5 publications with PCCA and Brain
 Nerve Antibodies  >2 publications with PCCA and Nerve
 Placenta Antibodies  >2 publications with PCCA and Placenta
 Embryonic Tissue Antibodies  >2 publications with PCCA and Embryonic Tissue
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