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SP3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3201457

This is a rabbit polyclonal antibody against SP3. It was validated on Western Blot using a cell lysate as a positive control.Target Description: SP3 is a transcriptional factor that can act as an activator or repressor, probably in a isoform-specific manner.… More details

$490 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SP3, Polyclonal Antibody
Full Product Name
SP3 antibody - middle region
Product Gene Name
Product Synonym Gene Name
DKFZp686O1631; SPR-2; SPR2
Immunogen Sequence
Synthetic peptide located within the following region: TAGINADGHL INTGQAMDSS DNSERTGERV SPDINETNTD TDLFVPTSSS
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
SP3 peptide (MBS3226473) is used for blocking the activity of SP3 antibody (MBS3201457)
Molecular Weight
82kDa
OMIM
601804
3D Structure
ModBase 3D Structure for Q02447
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human SP3
Applications Tested/Suitable for anti-SP3 antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-SP3 antibody

Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-SP3 antibody
Rabbit Anti-SP3 Polyclonal Antibody – Cat# MBS3201457

Western Blot (WB) of anti-SP3 antibody

Sample Type: Human U2OS
Sample Type: Human nuclear cell extracts (30ug)
Primary Dilution: 1:1000
Secondary Antibody: anti-Rabbit HRP
Secondary Dilution: 1:20000
Image Submitted By:  Katarina Luciakova
Cancer Research Institute See Customer Feedback tab for detailed information.

SP3 is supported by BioGPS gene expression data to be expressed in U2OS
Rabbit Anti-SP3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3201457

Western Blot (WB) of anti-SP3 antibody

Host: Mouse
Target Name: SP3
Sample Tissue: Mouse Skeletal Muscle
Antibody Dilution: 1ug/ml
Rabbit Anti-SP3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3201457

Western Blot (WB) of anti-SP3 antibody

WB Suggested Anti-SP3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Muscle
Rabbit Anti-SP3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3201457

Other Notes

Small volumes of anti-SP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SP3 purchase

MBS3201457 is an antibody targeting SP3. Also known as SP3 antibody - middle region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SP3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor Sp3 isoform 2
NCBI Official Synonym Full Names
Sp3 transcription factor
NCBI Official Symbol
SP3
NCBI Official Synonym Symbols
SPR2
UniProt Protein Name
Transcription factor Sp3
UniProt Synonym Protein Names
SPR-2
Protein Family
UniProt Gene Name
SP3
UniProt Entry Name
SP3_HUMAN
NCBI Protein Information
transcription factor Sp3

NCBI Summary for SP3

This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]
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UniProt Comments for SP3

Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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