• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

KLHL25 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3202172

This is a rabbit polyclonal antibody against KLHL25. It was validated on Western Blot and immunohistochemistryTarget Description: KLHL25 is also known as ENC2. It is a BTB/POZ KELCH domain protein More details

$440 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KLHL25, Polyclonal Antibody
Full Product Name
KLHL25 antibody - N-terminal region
Product Gene Name
anti-KLHL25 antibody
Product Synonym Gene Name
ENC2; FLJ12587; ENC-2
Immunogen Sequence
Synthetic peptide located within the following region: RLYEFSWRMC LVHFETVRQS EDFNSLSKDT LLDLISSDEL ETEDERVVFE
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Protein A purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
KLHL25 peptide (MBS3227156) is used for blocking the activity of KLHL25 antibody (MBS3202172)
Molecular Weight
66kDa
3D Structure
ModBase 3D Structure for Q9H0H3
Homology
Cow: 93%; Dog: 100%; Guinea Pig: 93%; Human: 100%; Mouse: 86%; Pig: 100%; Rabbit: 86%; Rat: 86%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human KLHL25

Product Categories/Family for anti-KLHL25 antibody

Polyclonal; Transcription Factor; Immunohistochemistry
Applications Tested/Suitable for anti-KLHL25 antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-KLHL25 antibody

Human Brain
Rabbit Anti-KLHL25 Polyclonal Antibody – Cat# MBS3202172

Western Blot (WB) of anti-KLHL25 antibody

WB Suggested Anti-KLHL25 Antibody Titration: 0.3125ug/ml
Positive Control: Jurkat cell lysate
Rabbit Anti-KLHL25 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3202172

Other Notes

Small volumes of anti-KLHL25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLHL25 purchase

MBS3202172 is an antibody targeting KLHL25. Also known as KLHL25 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KLHL25. It may not necessarily be applicable to this product.

NCBI GI #
21362105
NCBI GeneID
64410
NCBI Accession #
NP_071925
UniProt Primary Accession #
Q9H0H3
UniProt Related Accession #
Q9H0H3
GenBank Nucleotide #
NM_022480
NCBI Official Full Name
kelch-like protein 25
NCBI Official Synonym Full Names
kelch like family member 25
NCBI Official Symbol
KLHL25
NCBI Official Synonym Symbols
ENC2; ENC-2
UniProt Protein Name
Kelch-like protein 25
UniProt Synonym Protein Names
Ectoderm-neural cortex protein 2; ENC-2
Protein Family
Kelch-like protein
UniProt Gene Name
KLHL25
UniProt Synonym Gene Names
ENC2; ENC-2
UniProt Entry Name
KLH25_HUMAN
NCBI Protein Information
kelch-like protein 25

UniProt Comments for KLHL25

KLHL25: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E. Component of the BCR(KLHL25) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL25 and RBX1. Interacts with EIF4EBP1 (when hypophosphorylated)

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 15q25.3

Cellular Component: cytoplasm

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; regulation of translational initiation
Show more

Research Articles on KLHL25

1. CUL3 interacts with ACLY through its adaptor protein, KLHL25 (Kelch-like family member 25), to ubiquitinate and degrade ACLY in cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLHL25 antibody

 Reference Product  PubMed Publications
 EIF4EBP1 antibody  >1 publications with KLHL25 and EIF4EBP1
 CUL3 antibody  >1 publications with KLHL25 and CUL3
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000