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LIMA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3202308

This is a rabbit polyclonal antibody against LIMA1. It was validated on Western Blot using a cell lysate as a positive control.Target Description: EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links… More details

$490 / 0.1 mL +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
LIMA1, Polyclonal Antibody
Full Product Name
LIMA1 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
EPLIN; FLJ38853; MGC131726; SREBP3
Immunogen Sequence
Synthetic peptide located within the following region: PHIKDGEDLK DHSTESKKME NCLGESRHEV EKSEISENTD ASGKIEKYNV
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Horse, Human, Mouse, Rabbit, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
LIMA1 peptide (MBS3227287) is used for blocking the activity of LIMA1 antibody (MBS3202308)
Molecular Weight
85kDa
OMIM
608364
3D Structure
ModBase 3D Structure for Q9UHB6
Homology
Horse: 79%; Human: 100%; Mouse: 79%; Rabbit: 86%; Rat: 79%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human LIMA1
Applications Tested/Suitable for anti-LIMA1 antibody
Western Blot (WB)

Western Blot (WB) of anti-LIMA1 antibody

Host: Rabbit
Target Name: LIMA1
Sample Type: Human Fetal Heart
Antibody Dilution: 1.0ug/ml
Rabbit Anti-LIMA1 Polyclonal Antibody – Cat# MBS3202308

Western Blot (WB) of anti-LIMA1 antibody

Host: Rabbit
Target Name: LIMA1
Sample Type: Human Fetal Lung
Antibody Dilution: 1.0ug/ml
Rabbit Anti-LIMA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3202308

Western Blot (WB) of anti-LIMA1 antibody

WB Suggested Anti-LIMA1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Placenta
Rabbit Anti-LIMA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3202308

Other Notes

Small volumes of anti-LIMA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIMA1 purchase

MBS3202308 is an antibody targeting LIMA1. Also known as LIMA1 antibody - N-terminal region. Reactive in Horse, Human, Mouse, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LIMA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
LIM domain and actin-binding protein 1 isoform 2
NCBI Official Synonym Full Names
LIM domain and actin binding 1
NCBI Official Symbol
LIMA1
NCBI Official Synonym Symbols
EPLIN; LDLCQ8; SREBP3
UniProt Protein Name
LIM domain and actin-binding protein 1
UniProt Synonym Protein Names
Epithelial protein lost in neoplasm
UniProt Gene Name
LIMA1
UniProt Synonym Gene Names
EPLIN; SREBP3
UniProt Entry Name
LIMA1_HUMAN
NCBI Protein Information
LIM domain and actin-binding protein 1

NCBI Summary for LIMA1

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]
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UniProt Comments for LIMA1

eplin: Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: focal adhesion; cytoplasm; plasma membrane; stress fiber; cleavage furrow; actin cytoskeleton

Molecular Function: actin monomer binding; actin filament binding; protein binding; zinc ion binding

Biological Process: actin filament bundle formation; ruffle organization and biogenesis; negative regulation of actin filament depolymerization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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