Diphtheria Toxin Antibody – Mouse Monoclonal

Monoclonal

IgG1

Mouse

Purified

ModBase 3D Structure for P00588

Specific for the intact toxin/toxoid. Non-reactive with free A or B subunits. Functions in ELISA.

MONOTOPE purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations. The final preparation is formulated to a protein concentration of 100 ug/ml in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Each vial contains 1.0 ml. This product contains no stabilizing proteins and should be stored at -20°C until ready for use. Avoid repeated freeze-thawing by storing multiple aliquots at -20°C. Working dilution must be determined by the user. Suggested starting ranges are 1:10-1:50 for IFA and 1:20-1:200 for ELISA.

These MONOTOPE™ products consist of purified monoclonal antibody conjugated with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The final preparation is formulated to an antibody concentration of of 100 ug/ml in 0.01 M phosphate buffered saline, pH 7.2 containing 0.1% sodium azide plus bovine serum albumin at 10 mg/ml. Each vial contains 1.0 ml. This product should be stored at 2-8°C until ready for use. Avoid repeated freeze-thawing by storing multiple aliquots at -20°C. Applications for these products include direct FA staining of target antigen in a permissive tissue culture system. Working strength must be determined by the user for each specific application but a starting range of 1:5 - 1:20 is recommended. Acetone fixation ofthe antigen source is recommended prior to staining.