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DHX30 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3203170

This is a rabbit polyclonal antibody against DHX30. It was validated on Western Blot using a cell lysate as a positive control.Target Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They… More details

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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DHX30, Polyclonal Antibody
Full Product Name
DHX30 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
DDX30; FLJ11214; KIAA0890; RETCOR
Immunogen Sequence
Synthetic peptide located within the following region: AESGMAPGGP GEGDGSLVNA SRDLLKEFPQ PKNLLNSVIG RALGISHAKD
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Human, Mouse, Pig, Rabbit
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
DHX30 peptide (MBS3228143) is used for blocking the activity of DHX30 antibody (MBS3203170)
Molecular Weight
40kDa
3D Structure
ModBase 3D Structure for Q7L2E3
Homology
Cow: 93%; Dog: 93%; Human: 100%; Mouse: 93%; Pig: 93%; Rabbit: 93%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human DHX30
Applications Tested/Suitable for anti-DHX30 antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-DHX30 antibody

Rabbit Anti-DHX30 Antibody
Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver
Observed Staining: Cytoplasm in hepatocytes, moderate signal, wide tissue distribution
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Protocol located in Reviews and Data.
Rabbit Anti-DHX30 Polyclonal Antibody – Cat# MBS3203170

Immunohistochemistry (IHC) of anti-DHX30 antibody

Rabbit Anti-DHX30 Antibody
Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
Observed Staining: Cytoplasmic in alveolar type I cells
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Rabbit Anti-DHX30 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3203170

Western Blot (WB) of anti-DHX30 antibody

WB Suggested Anti-DHX30 Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysateDHX30 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Rabbit Anti-DHX30 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3203170

Other Notes

Small volumes of anti-DHX30 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DHX30 purchase

MBS3203170 is an antibody targeting DHX30. Also known as DHX30 antibody - N-terminal region. Reactive in Cow, Dog, Human, Mouse, Pig, Rabbit. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DHX30. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Synonym Full Names
DExH-box helicase 30
NCBI Official Symbol
DHX30
NCBI Official Synonym Symbols
DDX30; RETCOR; NEDMIAL
UniProt Protein Name
Putative ATP-dependent RNA helicase DHX30
UniProt Synonym Protein Names
DEAH box protein 30
UniProt Gene Name
DHX30
UniProt Synonym Gene Names
DDX30; KIAA0890
UniProt Entry Name
DHX30_HUMAN
NCBI Protein Information
ATP-dependent RNA helicase DHX30; putative ATP-dependent RNA helicase DHX30

NCBI Summary for DHX30

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]
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UniProt Comments for DHX30

DDX30: Required for optimal function of the zinc-finger antiviral protein ZC3HAV1. Associates with mitochondrial DNA. Belongs to the DEAD box helicase family. DEAH subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 3.6.4.13; RNA-binding; Helicase

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; double-stranded RNA binding; chromatin binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA processing
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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