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ATF3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3203183

This is a rabbit polyclonal antibody against ATF3. It was validated on Western Blot using a cell lysate as a positive control.Target Description: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many… More details

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Product Overview

Online Datasheet in PDF
Product Name
ATF3, Polyclonal Antibody
Full Product Name
ATF3 antibody - middle region
Product Gene Name
anti-ATF3 antibody
Immunogen Sequence
Synthetic peptide located within the following region: PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
ATF3 peptide (MBS3228156) is used for blocking the activity of ATF3 antibody (MBS3203183)
Molecular Weight
20kDa
OMIM
603148
3D Structure
ModBase 3D Structure for P18847
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 93%; Rat: 93%; Yeast: 75%; Zebrafish: 80%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human ATF3

Product Categories/Family for anti-ATF3 antibody

Polyclonal; Transcription Regulation; Transcription Regulation; DNA/RNA/Protein Interactions; Transcription Factors
Applications Tested/Suitable for anti-ATF3 antibody
Western Blot (WB)

Western Blot (WB) of anti-ATF3 antibody

Host: Rabbit
Target Name: ATF3
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml
Rabbit Anti-ATF3 Polyclonal Antibody – Cat# MBS3203183

Western Blot (WB) of anti-ATF3 antibody

Host: Rabbit
Target Name: ATF3
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 1ug/ml
Rabbit Anti-ATF3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3203183

Western Blot (WB) of anti-ATF3 antibody

Host: Rabbit
Target Name: ATF3
Sample Type: OVCAR-3
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 0.12
Rabbit Anti-ATF3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3203183

Western Blot (WB) of anti-ATF3 antibody

WB Suggested Anti-ATF3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: OVCAR-3 cell lysate
Rabbit Anti-ATF3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3203183

Other Notes

Small volumes of anti-ATF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATF3 purchase

MBS3203183 is an antibody targeting ATF3. Also known as ATF3 antibody - middle region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATF3. It may not necessarily be applicable to this product.

NCBI GI #
4502263
NCBI GeneID
467
NCBI Accession #
NP_001665
UniProt Primary Accession #
P18847
UniProt Related Accession #
P18847
GenBank Nucleotide #
NM_001674
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-3 isoform 1
NCBI Official Synonym Full Names
activating transcription factor 3
NCBI Official Symbol
ATF3
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-3
UniProt Synonym Protein Names
Activating transcription factor 3
Protein Family
Activating transcription factor
UniProt Gene Name
ATF3
UniProt Synonym Gene Names
cAMP-dependent transcription factor ATF-3
UniProt Entry Name
ATF3_HUMAN
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-3

NCBI Summary for ATF3

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]
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UniProt Comments for ATF3

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; gluconeogenesis
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Research Articles on ATF3

1. Study identified ATF3 as a tumor suppressor for inhibiting cell proliferation and metastasis in hepatocellular carcinoma (HCC) through targeting and regulating CYR61. Clinically, ATF3 was down-regulated in HCC tissues, and its expression significantly associated with intrahepatic metastases and overall survival.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATF3 antibody

 Reference Product  PubMed Publications
 JUN antibody  >204 publications with ATF3 and JUN
 ATF4 antibody  >71 publications with ATF3 and ATF4
 DDIT3 antibody  >62 publications with ATF3 and DDIT3
 EGR1 antibody  >42 publications with ATF3 and EGR1
 TP53 antibody  >16 publications with ATF3 and TP53
 KLF6 antibody  >5 publications with ATF3 and KLF6
 SMAD4 antibody  >4 publications with ATF3 and SMAD4
 PDX1 antibody  >2 publications with ATF3 and PDX1
 IFNG antibody  >1 publications with ATF3 and IFNG
 SMARCA4 antibody  >1 publications with ATF3 and SMARCA4

Pathways associated with anti-ATF3 antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hypertrophy Model Pathway antibodies  Hypertrophy Model Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 PERK Regulates Gene Expression Pathway antibodies  PERK Regulates Gene Expression Pathway Diagram

Diseases associated with anti-ATF3 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >149 publications with ATF3 and Nervous System Diseases
 Disease Models, Animal Antibodies  >119 publications with ATF3 and Disease Models, Animal
 Pain Antibodies  >109 publications with ATF3 and Pain
 Inflammation Antibodies  >103 publications with ATF3 and Inflammation
 Peripheral Nervous System Diseases Antibodies  >72 publications with ATF3 and Peripheral Nervous System Diseases
 Necrosis Antibodies  >53 publications with ATF3 and Necrosis
 Cardiovascular Diseases Antibodies  >51 publications with ATF3 and Cardiovascular Diseases
 Lung Diseases Antibodies  >28 publications with ATF3 and Lung Diseases
 Nerve Degeneration Antibodies  >26 publications with ATF3 and Nerve Degeneration
 Liver Diseases Antibodies  >26 publications with ATF3 and Liver Diseases

Organs/Tissues associated with anti-ATF3 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >168 publications with ATF3 and Nerve
 Ganglia Antibodies  >124 publications with ATF3 and Ganglia
 Brain Antibodies  >91 publications with ATF3 and Brain
 Blood Antibodies  >88 publications with ATF3 and Blood
 Liver Antibodies  >66 publications with ATF3 and Liver
 Muscle Antibodies  >60 publications with ATF3 and Muscle
 Bone Antibodies  >49 publications with ATF3 and Bone
 Lung Antibodies  >46 publications with ATF3 and Lung
 Vascular Antibodies  >44 publications with ATF3 and Vascular
 Skin Antibodies  >43 publications with ATF3 and Skin
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