Rabbit Anti-PIAS2 Polyclonal Antibody – Cat# MBS3203794

WB Suggested Anti-Pias2 Antibody Titration: 0.2-1 ug/ml
Positive Control: Mouse Thymus

PIAS2 Antibody – Rabbit Polyclonal

Name: PIAS2 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS3203794
Price: 490 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3203794

This is a rabbit polyclonal antibody against Pias2. It was validated on Western BlotTarget Description: Pias2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a… More details

$490 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Pias2, Polyclonal Antibody

Full Product Name

Pias2 antibody - N-terminal region

Product Gene Name

anti-PIAS2 antibody

Product Synonym Gene Name

6330408K17Rik; AI462206; ARIP3; AU018068; Dib; Miz1; PIASxalpha; PIASxb; PIASxbeta; SIZ2

Immunogen Sequence

Synthetic peptide located within the following region: SVFSLDGSSS PVEPDLPVAG IHSLPSTSIT PHSPSSPVGS VLLQDTKPTF

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Form/Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Purity/Purification

Affinity Purified

Preparation and Storage

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Antibody/Peptide Pairs

Pias2 peptide (MBS3228762) is used for blocking the activity of Pias2 antibody (MBS3203794)

Molecular Weight

68kDa

3D Structure

ModBase 3D Structure for Q8C5D8

Homology

Cow: 86%; Dog: 86%; Guinea Pig: 86%; Horse: 93%; Human: 93%; Mouse: 100%; Pig: 93%; Rabbit: 79%; Rat: 93%

Immunogen

The immunogen is a synthetic peptide corresponding to a region of Mouse

Product Categories/Family for anti-PIAS2 antibody

Polyclonal; Transcription Factor; Transcription Regulation; Cell Biology; Sumoylation; Transcription Factors

Applications Tested/Suitable for anti-PIAS2 antibody

Western Blot (WB)

Other Notes

Small volumes of anti-PIAS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIAS2 purchase

MBS3203794 is an antibody targeting PIAS2. Also known as Pias2 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PIAS2. It may not necessarily be applicable to this product.

NCBI GI #

255918212

NCBI GeneID

17344

NCBI Accession #

NP_032628

UniProt Primary Accession #

Q8C5D8

UniProt Related Accession #

Q8C5D8

GenBank Nucleotide #

NM_008602

NCBI Official Full Name

E3 SUMO-protein ligase PIAS2 isoform 1

NCBI Official Synonym Full Names

protein inhibitor of activated STAT 2

NCBI Official Symbol

Pias2

NCBI Official Synonym Symbols

Dib; Miz1; SIZ2; ARIP3; PIASxb; AI462206; AU018068; PIASxbeta; PIASxalpha; 6330408K17Rik

UniProt Protein Name

E3 SUMO-protein ligase PIAS2

UniProt Synonym Protein Names

Androgen receptor-interacting protein 3; ARIP3; DAB2-interacting protein; DIP; Msx-interacting zinc finger protein; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2

Protein Family

E3 SUMO-protein ligase

UniProt Gene Name

Pias2

UniProt Synonym Gene Names

Miz1; Piasx; ARIP3; DIP

UniProt Entry Name

PIAS2_MOUSE

NCBI Protein Information

E3 SUMO-protein ligase PIAS2

UniProt Comments for PIAS2

PIAS2: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2- alpha. Belongs to the PIAS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: SUMO conjugating system; Nuclear receptor co-regulator; EC 6.3.2.-; Transcription, coactivator/corepressor

Cellular Component: nuclear body; PML body; nucleus

Molecular Function: protein domain specific binding; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; ribosomal S6-glutamic acid ligase activity; SUMO ligase activity; transcription factor binding; protein binding; DNA binding; androgen receptor binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin protein ligase binding; estrogen receptor binding; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity; glucocorticoid receptor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; protein sumoylation; regulation of osteoblast differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of dendrite morphogenesis

Research Articles on PIAS2

1. A direct interaction between Pold2 and PIAS2 was confirmed by direct yeast two-hybrid.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.