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GATA4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3203897

This is a rabbit polyclonal antibody against GATA4. It was validated on Western Blot using a cell lysate as a positive control.Target Description: GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the… More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GATA4, Polyclonal Antibody
Full Product Name
GATA4 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
ASD2; VSD1
Immunogen Sequence
Synthetic peptide located within the following region: MYQSLAMAAN HGPPPGAYEA GGPGAFMHGA GAASSPVYVP TPRVPSSVLG
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Human, Mouse, Pig, Rabbit, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
GATA4 peptide (MBS3228866) is used for blocking the activity of GATA4 antibody (MBS3203897)
Molecular Weight
45kDa
3D Structure
ModBase 3D Structure for Q5IFM8
Homology
Cow: 93%; Dog: 100%; Human: 100%; Mouse: 100%; Pig: 93%; Rabbit: 100%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human GATA4
Applications Tested/Suitable for anti-GATA4 antibody
Western Blot (WB)

Western Blot (WB) of anti-GATA4 antibody

Lanes:
1: 25 ug mouse tumor + shRNA1, 2: 25 ug mouse tumor + shRNA2, 3: 25 ug mouse tumor + shRNA3 4: 25 ug mouse tumor lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:3500
Gene Name:
GATA4
Submitted by:
Dr. Jonahan Kurie, UT MD Anderson Cancer center
Rabbit Anti-GATA4 Polyclonal Antibody – Cat# MBS3203897

Western Blot (WB) of anti-GATA4 antibody

WB Suggested Anti-GATA4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate
Rabbit Anti-GATA4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3203897

Other Notes

Small volumes of anti-GATA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GATA4 purchase

MBS3203897 is an antibody targeting GATA4. Also known as GATA4 antibody - N-terminal region. Reactive in Cow, Dog, Human, Mouse, Pig, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GATA4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor GATA-4 isoform 2
NCBI Official Synonym Full Names
GATA binding protein 4
NCBI Official Symbol
GATA4
NCBI Official Synonym Symbols
TOF; ASD2; VSD1; TACHD
NCBI Protein Information
transcription factor GATA-4

NCBI Summary for GATA4

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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