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PLD2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3203948

This is a rabbit polyclonal antibody against PLD2. It was validated on Western Blot using a cell lysate as a positive control.Target Description: Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce… More details

$490 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PLD2, Polyclonal Antibody
Full Product Name
PLD2 antibody - N-terminal region
Product Gene Name
Immunogen Sequence
Synthetic peptide located within the following region: MTATPESLFP TGDELDSSQL QMESDEVDTL KEGEDPADRM HPFLAIYELQ
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
PLD2 peptide (MBS3228917) is used for blocking the activity of PLD2 antibody (MBS3203948)
Molecular Weight
106kDa
OMIM
602384
3D Structure
ModBase 3D Structure for O14939
Homology
Cow: 86%; Dog: 85%; Guinea Pig: 83%; Horse: 86%; Human: 100%; Mouse: 75%; Rabbit: 86%; Rat: 75%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human PLD2
Applications Tested/Suitable for anti-PLD2 antibody
Western Blot (WB)

Western Blot (WB) of anti-PLD2 antibody

WB Suggested Anti-PLD2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Transfected 293T
Rabbit Anti-PLD2 Polyclonal Antibody – Cat# MBS3203948

Other Notes

Small volumes of anti-PLD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLD2 purchase

MBS3203948 is an antibody targeting PLD2. Also known as PLD2 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PLD2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phospholipase D2 isoform PLD2A
NCBI Official Synonym Full Names
phospholipase D2
NCBI Official Symbol
PLD2
NCBI Official Synonym Symbols
PLD1C
UniProt Protein Name
Phospholipase D2
UniProt Synonym Protein Names
Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2
Protein Family
UniProt Gene Name
PLD2
UniProt Synonym Gene Names
PLD 2; hPLD2
UniProt Entry Name
PLD2_HUMAN
NCBI Protein Information
phospholipase D2

NCBI Summary for PLD2

The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
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UniProt Comments for PLD2

PLD2: a phosphatidylcholine-specific phospholipase D. Catalyzes the hydrolysis of PC to produce phosphatidic acid and choline. Activated as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. May function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control. Enriched in caveolae; may be involved in MEK/ERK signaling cascades induced by the VEGF/VEGFR-2/PKC-delta pathway in endothelial cells. Three alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.1.4.4; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Phospholipase

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: endoplasmic reticulum membrane; brush border membrane; plasma membrane

Molecular Function: protein binding; phospholipase D activity; phosphoinositide binding

Biological Process: G-protein coupled receptor internalization; phosphatidylglycerol biosynthetic process; small GTPase mediated signal transduction; phospholipid metabolic process; glycerophospholipid biosynthetic process; innate immune response; phosphatidic acid biosynthetic process; cytoskeleton organization and biogenesis; lipid catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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