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ZMIZ1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3204610

This is a rabbit polyclonal antibody against ZMIZ1. It was validated on Western Blot using a cell lysate as a positive control.Target Description: ZMIZ1 increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of… More details

$490 / 0.1 mL +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ZMIZ1, Polyclonal Antibody
Full Product Name
ZMIZ1 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
FLJ13541; KIAA1224; MIZ; RAI17; Zimp10; hZIMP10; ZIMP10; TRAFIP10
Immunogen Sequence
Synthetic peptide located within the following region: MNSMDRHIQQ TNDRLQCIKQ HLQNPANFHN AATELLDWCG DPRAFQRPFE
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
ZMIZ1 peptide (MBS3229577) is used for blocking the activity of ZMIZ1 antibody (MBS3204610)
Molecular Weight
115kDa
OMIM
607159
3D Structure
ModBase 3D Structure for Q9ULJ6
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 82%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human ZMIZ1
Applications Tested/Suitable for anti-ZMIZ1 antibody
Western Blot (WB)

Western Blot (WB) of anti-ZMIZ1 antibody

Lanes:
Lane 1: 50ug mouse retina extract
Lane 2: 50ug mouse prostate extract
Lane 3: 50ug mouse testis extract
Lane 4: 50ug mouse colon extract
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
ZMIZ1
Submitted by:
John Fu, UVA
Rabbit Anti-ZMIZ1 Polyclonal Antibody – Cat# MBS3204610

Western Blot (WB) of anti-ZMIZ1 antibody

WB Suggested Anti-ZMIZ1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: 293T cell lysate
Rabbit Anti-ZMIZ1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3204610

Other Notes

Small volumes of anti-ZMIZ1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZMIZ1 purchase

MBS3204610 is an antibody targeting ZMIZ1. Also known as ZMIZ1 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ZMIZ1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
zinc finger MIZ domain-containing protein 1
NCBI Official Synonym Full Names
zinc finger MIZ-type containing 1
NCBI Official Symbol
ZMIZ1
NCBI Official Synonym Symbols
MIZ; RAI17; ZIMP10; TRAFIP10
UniProt Protein Name
Zinc finger MIZ domain-containing protein 1
UniProt Synonym Protein Names
PIAS-like protein Zimp10; Retinoic acid-induced protein 17
UniProt Gene Name
ZMIZ1
UniProt Synonym Gene Names
KIAA1224; RAI17; ZIMP10
UniProt Entry Name
ZMIZ1_HUMAN
NCBI Protein Information
zinc finger MIZ domain-containing protein 1

NCBI Summary for ZMIZ1

This gene encodes a member of the PIAS (protein inhibitor of activated STAT) family of proteins. The encoded protein regulates the activity of various transcription factors, including the androgen receptor, Smad3/4, and p53. The encoded protein may also play a role in sumoylation. A translocation between this locus on chromosome 10 and the protein tyrosine kinase ABL1 locus on chromosome 9 has been associated with acute lymphoblastic leukemia. [provided by RefSeq, Mar 2010]
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UniProt Comments for ZMIZ1

RAI17: Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear speck

Molecular Function: zinc ion binding

Biological Process: heart morphogenesis; developmental growth; positive regulation of fibroblast proliferation; transcription, DNA-dependent; in utero embryonic development; vitellogenesis; artery morphogenesis; cell aging; positive regulation of transcription from RNA polymerase II promoter; vasculogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ZMIZ1 antibody

 Reference Product  PubMed Publications
 SMAD4 antibody  >1 publications with ZMIZ1 and SMAD4
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