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PUF60 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3205458

This is a rabbit polyclonal antibody against PUF60. It was validated on Western Blot and immunohistochemistryTarget Description: PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for… More details

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Product Overview

Online Datasheet in PDF
Product Name
PUF60, Polyclonal Antibody
Full Product Name
PUF60 antibody - C-terminal region
Product Gene Name
anti-PUF60 antibody
Product Synonym Gene Name
FIR; FLJ31379; RoBPI; SIAHBP1
Immunogen Sequence
Synthetic peptide located within the following region: EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Protein A purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
PUF60 peptide (MBS3230423) is used for blocking the activity of PUF60 antibody (MBS3205458)
Molecular Weight
58kDa
OMIM
gene 615583
3D Structure
ModBase 3D Structure for Q9UHX1
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the C terminal region of human PUF60

Product Categories/Family for anti-PUF60 antibody

Polyclonal; Signal Proteins; Lymphocyte Signaling; Membrane Protein; Transcription Regulation; Developmental Biology; Immunohistochemistry; Receptors; Transcription Factors
Applications Tested/Suitable for anti-PUF60 antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-PUF60 antibody

Sample Type: Human Intestine
Rabbit Anti-PUF60 Antibody Paraffin Embedded Tissue: Human Intestine Cellular Data: Epithelial cells of intestinal villas Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X
Rabbit Anti-PUF60 Polyclonal Antibody – Cat# MBS3205458

Immunohistochemistry (IHC) of anti-PUF60 antibody

Rabbit Anti-PUF60 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3205458

Western Blot (WB) of anti-PUF60 antibody

WB Suggested Anti-PUF60 Antibody
Titration: 0.5ug/ml
Positive Control: Human 293T Cell Lysate
Rabbit Anti-PUF60 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3205458

Western Blot (WB) of anti-PUF60 antibody

WB Suggested Anti-PUF60 Antibody Titration: 0.2-1 ug/mlPositive Control: HelaPUF60 is supported by BioGPS gene expression data to be expressed in HeLa
Rabbit Anti-PUF60 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3205458

Other Notes

Small volumes of anti-PUF60 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PUF60 purchase

MBS3205458 is an antibody targeting PUF60. Also known as PUF60 antibody - C-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PUF60. It may not necessarily be applicable to this product.

NCBI GI #
17298690
NCBI GeneID
22827
NCBI Accession #
NP_055096
UniProt Primary Accession #
Q9UHX1
UniProt Related Accession #
Q9UHX1
GenBank Nucleotide #
NM_014281
NCBI Official Full Name
poly(U)-binding-splicing factor PUF60 isoform b
NCBI Official Synonym Full Names
poly(U) binding splicing factor 60
NCBI Official Symbol
PUF60
NCBI Official Synonym Symbols
FIR; VRJS; RoBPI; SIAHBP1
UniProt Protein Name
Poly(U)-binding-splicing factor PUF60
UniProt Synonym Protein Names
60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1
Protein Family
Poly(U)-binding-splicing factor
UniProt Gene Name
PUF60
UniProt Synonym Gene Names
FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1
UniProt Entry Name
PUF60_HUMAN
NCBI Protein Information
poly(U)-binding-splicing factor PUF60

NCBI Summary for PUF60

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
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UniProt Comments for PUF60

FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; ribonucleoprotein complex; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: identical protein binding; protein binding; DNA binding; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; RNA splicing; mRNA processing

Disease: Verheij Syndrome
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Research Articles on PUF60

1. Anti-PUF60 antibodies were nonspecific for myositis, since they could be detected in other rheumatic diseases.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PUF60 antibody

 Products by Pathway  Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram

Diseases associated with anti-PUF60 antibody

 Disease Name  Pubmed Publications
 Ovarian Diseases Antibodies  >1 publications with PUF60 and Ovarian Diseases
 Neoplasm Metastasis Antibodies  >1 publications with PUF60 and Neoplasm Metastasis

Organs/Tissues associated with anti-PUF60 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >1 publications with PUF60 and Heart
 Stomach Antibodies  >1 publications with PUF60 and Stomach
 Lymph Antibodies  >1 publications with PUF60 and Lymph
 Lymph Node Antibodies  >1 publications with PUF60 and Lymph Node
 Intestine Antibodies  >1 publications with PUF60 and Intestine
 Ovary Antibodies  >1 publications with PUF60 and Ovary
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