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KHK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3205920

This is a rabbit polyclonal antibody against KHK. It was validated on Western Blot using a cell lysate as a positive control.Target Description: KHK is a ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is… More details

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Product Overview

Online Datasheet in PDF
Product Name
KHK, Polyclonal Antibody
Full Product Name
KHK antibody - N-terminal region
Product Gene Name
Immunogen Sequence
Synthetic peptide located within the following region: FLVADFRRRG VDVSQVAWQS KGDTPSSCCI INNSNGNRTI VLHDTSLPDV
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Human
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
KHK peptide (MBS3230882) is used for blocking the activity of KHK antibody (MBS3205920)
Molecular Weight
32kDa
OMIM
229800
3D Structure
ModBase 3D Structure for P50053
Homology
Cow: 79%; Dog: 79%; Guinea Pig: 79%; Human: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human KHK
Applications Tested/Suitable for anti-KHK antibody
Western Blot (WB)

Immunohistochemistry (IHC) of anti-KHK antibody

Rabbit Anti-KHK antibody
Formalin Fixed Paraffin Embedded Tissue: Human Kidney
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec
Rabbit Anti-KHK Polyclonal Antibody – Cat# MBS3205920

Western Blot (WB) of anti-KHK antibody

WB Suggested Anti-KHK Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Small Intestine
Rabbit Anti-KHK Polyclonal Antibody, Western Blot (WB) – Cat# MBS3205920

Other Notes

Small volumes of anti-KHK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KHK purchase

MBS3205920 is an antibody targeting KHK. Also known as KHK antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KHK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ketohexokinase isoform b
NCBI Official Synonym Full Names
ketohexokinase
NCBI Official Symbol
KHK
UniProt Protein Name
Ketohexokinase
UniProt Synonym Protein Names
Hepatic fructokinase
Protein Family
UniProt Gene Name
KHK
UniProt Entry Name
KHK_HUMAN
NCBI Protein Information
ketohexokinase

NCBI Summary for KHK

This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
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UniProt Comments for KHK

KHK iso2: Defects in KHK are the cause of fructosuria (FRUCT). Benign defect of intermediary metabolism. Belongs to the carbohydrate kinase PfkB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.3; Carbohydrate Metabolism - fructose and mannose; Transferase

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: ketohexokinase activity; ATP binding

Biological Process: carbohydrate phosphorylation; carbohydrate metabolic process; pathogenesis; fructose catabolic process

Disease: Fructosuria, Essential
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KHK antibody

 Reference Product  PubMed Publications
 HK1 antibody  >1 publications with KHK and HK1
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