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HSD3B1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3206056

This is a rabbit polyclonal antibody against HSD3B1. It was validated on Western Blot using a cell lysate as a positive control.Target Description: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy… More details

$490 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HSD3B1, Polyclonal Antibody
Full Product Name
HSD3B1 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
HSD3B; HSDB3; I; HSDB3A; SDR11E1; 3BETAHSD
Immunogen Sequence
Synthetic peptide located within the following region: TGWSCLVTGA GGFLGQRIIR LLVKEKELKE IRVLDKAFGP ELREEFSKLQ
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Goat, Horse, Human, Pig, Rat, Sheep, Monkey
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
HSD3B1 peptide (MBS3231019) is used for blocking the activity of HSD3B1 antibody (MBS3206056)
Molecular Weight
42kDa
OMIM
109715
3D Structure
ModBase 3D Structure for P14060
Homology
Cow: 79%; Goat: 82%; Horse: 79%; Human: 100%; Pig: 79%; Rat: 79%; Sheep: 79%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human HSD3B1
Applications Tested/Suitable for anti-HSD3B1 antibody
Western Blot (WB)

Western Blot (WB) of anti-HSD3B1 antibody

Sample Type: Monkey brain extract
Lanes :
Lane 1: 50ug monkey brain extract
Primary Antibody Dilution :
1:1000
Secondary Antibody :
Goat anti rabbit-HRP
Secondary Antibody Dilution :
1:10,000
Gene Name :
HSD3B1
Submitted by :
Jonathan Bertin, Endoceutics Inc.
Rabbit Anti-HSD3B1 Polyclonal Antibody – Cat# MBS3206056

Western Blot (WB) of anti-HSD3B1 antibody

Host: Rabbit
Target Name: HSD3B1
Sample Type: Human Adult Placenta
Antibody Dilution: 1.0ug/ml
Rabbit Anti-HSD3B1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3206056

Western Blot (WB) of anti-HSD3B1 antibody

WB Suggested Anti-HSD3B1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human Placenta
Rabbit Anti-HSD3B1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3206056

Other Notes

Small volumes of anti-HSD3B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSD3B1 purchase

MBS3206056 is an antibody targeting HSD3B1. Also known as HSD3B1 antibody - N-terminal region. Reactive in Cow, Goat, Horse, Human, Pig, Rat, Sheep, Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HSD3B1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
NCBI Official Symbol
HSD3B1
NCBI Official Synonym Symbols
HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
UniProt Synonym Protein Names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; Trophoblast antigen FDO161GIncluding the following 2 domains:3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)Alternative name(s):3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase
UniProt Gene Name
HSD3B1
UniProt Synonym Gene Names
3BH; HSDB3A; 3-beta-HSD I
UniProt Entry Name
3BHS1_HUMAN
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

NCBI Summary for HSD3B1

The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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