Rabbit Anti-MARCH8 Polyclonal Antibody – Cat# MBS3206634

WB Suggested Anti-MARCH8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: PANC1 cell lysateMARCH8 is supported by BioGPS gene expression data to be expressed in PANC1

MARCH8 Antibody – Rabbit Polyclonal

Name: MARCH8 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3206634
Price: 490 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3206634

Target Description: MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane… More details

$490 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MARCH8, Polyclonal Antibody

Full Product Name

MARCH8 Antibody - N-terminal region

Product Gene Name

anti-MARCH8 antibody

Product Synonym Gene Name

MARCH-VIII; MIR; RNF178; c-MIR

Immunogen Sequence

Synthetic peptide located within the following region: MSMPLHQISA IPSQDAISAR VYRSKTKEKE REEQNEKTLG HFMSHSSNIS

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog

Form/Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Purity/Purification

Affinity Purified

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

For short term use, store at 2°C to 8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Antibody/Peptide Pairs

MARCH8 peptide (MBS3231598) is used for blocking the activity of MARCH8 antibody (MBS3206634)

Molecular Weight

33kDa

OMIM

613335

3D Structure

ModBase 3D Structure for Q5T0T0

Predicted Homology

Cow: 93%; Dog: 79%; Human: 100%; Mouse: 100%; Rat: 100%

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human MARCH8

Protein Size (# AA)

291 amino acids

Protein Interactions

TNFRSF10A; UBC; UBE2H; UBE2D1; TFRC; UBE2D3; IL1RAP; IL1R1; CD86;

Blocking Peptide

For anti-MARCH8 (MBS3206634) antibody is Catalog # MBS3231598

Sample Type Confirmation

MARCH8 is supported by BioGPS gene expression data to be expressed in PANC1

Product Categories/Family for anti-MARCH8 antibody

Polyclonal; Membrane Protein; E3 & Ubiquitin; Membrane & Traffic

Applications Tested/Suitable for anti-MARCH8 antibody

Western Blot (WB)

Other Notes

Small volumes of anti-MARCH8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MARCH8 purchase

MBS3206634 is an antibody targeting MARCH8. Also known as MARCH8 Antibody - N-terminal region. Reactive in Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MARCH8. It may not necessarily be applicable to this product.

NCBI GI #

50539410

NCBI GeneID

220972

NCBI Accession #

NP_001002265

UniProt Primary Accession #

Q5T0T0

UniProt Related Accession #

Q5T0T0

GenBank Nucleotide #

NM_001002265

NCBI Official Full Name

E3 ubiquitin-protein ligase MARCH8 isoform a

NCBI Official Synonym Full Names

membrane associated ring-CH-type finger 8

NCBI Official Symbol

MARCH8

NCBI Official Synonym Symbols

MIR; CMIR; c-MIR; RNF178; MARCH-VIII

UniProt Protein Name

E3 ubiquitin-protein ligase MARCH8

UniProt Synonym Protein Names

Cellular modulator of immune recognition; c-MIR; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; MARCH-VIII; RING finger protein 178

Protein Family

E3 ubiquitin-protein ligase

UniProt Gene Name

MARCH8

UniProt Synonym Gene Names

MIR; RNF178; c-MIR; MARCH-VIII

UniProt Entry Name

MARH8_HUMAN

NCBI Protein Information

E3 ubiquitin-protein ligase MARCH8

NCBI Summary for MARCH8

MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]

UniProt Comments for MARCH8

MARCH8: E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS.

Protein type: Membrane protein, multi-pass; Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: cytoplasmic vesicle membrane; lysosomal membrane; early endosome membrane; lysosome; integral to membrane; endosome

Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; MHC class II protein binding; ligase activity

Biological Process: protein polyubiquitination; antigen processing and presentation of peptide antigen via MHC class II; negative regulation of MHC class II biosynthetic process

Research Articles on MARCH8

1. Knockdown of MARCH8 inactivated the PI3K and ss-catenin stat3 signaling pathways by changing protein expression levels or the phosphorylation of related proteins and inhibited migration and invasion of MKN-45 and AGS cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MARCH8 antibody

Reference Product	PubMed Publications
CD86 antibody	>2 publications with MARCH8 and CD86

Diseases associated with anti-MARCH8 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>2 publications with MARCH8 and Necrosis
Inflammation Antibodies	>1 publications with MARCH8 and Inflammation

Organs/Tissues associated with anti-MARCH8 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3 publications with MARCH8 and Blood
Heart Antibodies	>1 publications with MARCH8 and Heart
Bone Antibodies	>1 publications with MARCH8 and Bone
Lung Antibodies	>1 publications with MARCH8 and Lung
Bone Marrow Antibodies	>1 publications with MARCH8 and Bone Marrow