• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

UBE2M Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3206671

This is a rabbit polyclonal antibody against UBE2M. It was validated on Western Blot using a cell lysate as a positive control.Target Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or… More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UBE2M, Polyclonal Antibody
Full Product Name
UBE2M antibody - N-terminal region
Product Gene Name
anti-UBE2M antibody
Product Synonym Gene Name
UBC-RS2; UBC12; hUbc12
Immunogen Sequence
Synthetic peptide located within the following region: IKLFSLKQQK KEEESAGGTK GSSKKASAAQ LRIQKDINEL NLPKTCDISF
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
UBE2M peptide (MBS3231635) is used for blocking the activity of UBE2M antibody (MBS3206671)
Molecular Weight
21kDa
OMIM
603173
3D Structure
ModBase 3D Structure for P61081
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2M

Product Categories/Family for anti-UBE2M antibody

Polyclonal; E3 & Ubiquitin; Immunohistochemistry
Applications Tested/Suitable for anti-UBE2M antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-UBE2M antibody

Sample Type: Mouse Brain
Sample Type:NT2 cells
Red:Antibody
Blue:DAPI
Primary Dilution:1ug/50ul antibody
Secondary Antibody:Alexa goat anti-rabbit 594
Image Submitted by:Yuzhi Chen, University of Arkansas for Medical Sciences
Rabbit Anti-UBE2M Polyclonal Antibody – Cat# MBS3206671

Immunohistochemistry (IHC) of anti-UBE2M antibody

Sample Type :
Human brain stem cells
Primary Antibody Dilution :
1:500
Secondary Antibody :
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution :
1:1000
Color/Signal Descriptions :
UBE2M: Red DAPI:Blue
Gene Name :
UBE2M
Submitted by :
Dr. Yuzhi Chen, University of Arkansas for Medical Science
Rabbit Anti-UBE2M Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3206671

Western Blot (WB) of anti-UBE2M antibody

Sample Type: Mouse Brain
Sample Type:2. mouse brain extracts (80ug)
Primary Antibody Dilution:2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution:1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science
Rabbit Anti-UBE2M Polyclonal Antibody, Western Blot (WB) – Cat# MBS3206671

Western Blot (WB) of anti-UBE2M antibody

Host: Rabbit
Target Name: UBE2M
Sample Type: Human 721_B
Antibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in 721_B
Rabbit Anti-UBE2M Polyclonal Antibody, Western Blot (WB) – Cat# MBS3206671

Western Blot (WB) of anti-UBE2M antibody

Host: Rabbit
Target Name: UBE2M
Sample Type: Human MCF7
Antibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in MCF7
Rabbit Anti-UBE2M Polyclonal Antibody, Western Blot (WB) – Cat# MBS3206671

Western Blot (WB) of anti-UBE2M antibody

WB Suggested Anti-UBE2M Antibody Titration: 0.2-1 ug/ml
Positive Control: 721_B cell lysateUBE2M is supported by BioGPS gene expression data to be expressed in 721_B
Rabbit Anti-UBE2M Polyclonal Antibody, Western Blot (WB) – Cat# MBS3206671

Other Notes

Small volumes of anti-UBE2M antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBE2M purchase

MBS3206671 is an antibody targeting UBE2M. Also known as UBE2M antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2M. It may not necessarily be applicable to this product.

NCBI GI #
4507791
NCBI GeneID
9040
NCBI Accession #
NP_003960
UniProt Primary Accession #
P61081
UniProt Related Accession #
P61081
GenBank Nucleotide #
NM_003969
NCBI Official Full Name
NEDD8-conjugating enzyme Ubc12
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 M
NCBI Official Symbol
UBE2M
NCBI Official Synonym Symbols
UBC12; hUbc12; UBC-RS2
UniProt Protein Name
NEDD8-conjugating enzyme Ubc12
UniProt Synonym Protein Names
NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M
UniProt Gene Name
UBE2M
UniProt Synonym Gene Names
UBC12
UniProt Entry Name
UBC12_HUMAN
NCBI Protein Information
NEDD8-conjugating enzyme Ubc12

NCBI Summary for UBE2M

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for UBE2M

UBE2M: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. Interacts with UBA3, DCUN1D1 and RBX1. Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.

Protein type: Ubiquitin ligase; Ligase; Adaptor/scaffold; Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; ATP binding; NEDD8 ligase activity

Biological Process: protein neddylation; positive regulation of neuron apoptosis; transforming growth factor beta receptor signaling pathway; protein modification process; protein ubiquitination
Show more

Research Articles on UBE2M

1. the DCN1-UBC12 interaction is inhibited by DI-591, a high-affinity, cell-permeable small-molecule inhibitor that binds to purified recombinant human DCN1 and DCN2 proteins with K i values of 10-12 nM

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UBE2M antibody

 Reference Product  PubMed Publications
 NEDD8 antibody  >4 publications with UBE2M and NEDD8
 RBX1 antibody  >2 publications with UBE2M and RBX1
 CUL5 antibody  >1 publications with UBE2M and CUL5
 CUL1 antibody  >1 publications with UBE2M and CUL1

Pathways associated with anti-UBE2M antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Loss Of Function Of SMAD2/3 In Cancer Pathway antibodies  Loss Of Function Of SMAD2/3 In Cancer Pathway Diagram
 Loss Of Function Of SMAD4 In Cancer Pathway antibodies  Loss Of Function Of SMAD4 In Cancer Pathway Diagram
 Loss Of Function Of TGFBR1 In Cancer Pathway antibodies  Loss Of Function Of TGFBR1 In Cancer Pathway Diagram
 Loss Of Function Of TGFBR2 In Cancer Pathway antibodies  Loss Of Function Of TGFBR2 In Cancer Pathway Diagram
 SMAD2/3 MH2 Domain Mutants In Cancer Pathway antibodies  SMAD2/3 MH2 Domain Mutants In Cancer Pathway Diagram

Diseases associated with anti-UBE2M antibody

 Disease Name  Pubmed Publications
 Movement Disorders Antibodies  >1 publications with UBE2M and Movement Disorders
 Brain Diseases Antibodies  >1 publications with UBE2M and Brain Diseases
 Urinary Bladder Neoplasms Antibodies  >1 publications with UBE2M and Urinary Bladder Neoplasms

Organs/Tissues associated with anti-UBE2M antibody

 Organ/Tissue Name  Pubmed Publications
 Bladder Antibodies  >1 publications with UBE2M and Bladder
 Brain Antibodies  >1 publications with UBE2M and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000