• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

RHOT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3207541

This is a rabbit polyclonal antibody against RHOT1. It was validated on Western Blot and immunohistochemistryTarget Description: Mitochondrial GTPase involved in mitochondrial trafficking. RHOT1 is probably involved in control of anterograde transport of… More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RHOT1, Polyclonal Antibody
Full Product Name
RHOT1 antibody - N-terminal region
Product Gene Name
anti-RHOT1 antibody
Product Synonym Gene Name
ARHT1; FLJ11040; FLJ12633; MIRO-1; MIRO1
Immunogen Sequence
Synthetic peptide located within the following region: MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
RHOT1 peptide (MBS3232506) is used for blocking the activity of RHOT1 antibody (MBS3207541)
Molecular Weight
71kDa
OMIM
613888
3D Structure
ModBase 3D Structure for Q8IXI2
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human RHOT1

Product Categories/Family for anti-RHOT1 antibody

Polyclonal; Signal Proteins; Lymphocyte Signaling; Membrane Protein; Developmental Biology; Immunohistochemistry; Receptors
Applications Tested/Suitable for anti-RHOT1 antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-RHOT1 antibody

Rabbit Anti-RHOT1 Antibody
Paraffin Embedded Tissue: Human Muscle
Cellular Data: Skeletal muscle cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Rabbit Anti-RHOT1 Polyclonal Antibody – Cat# MBS3207541

Western Blot (WB) of anti-RHOT1 antibody

Host: Rabbit
Target Name: MIRO1
Sample Type: Fetal Lung lysates
Antibody Dilution: 1.0ug/ml
Rabbit Anti-RHOT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3207541

Western Blot (WB) of anti-RHOT1 antibody

Host: Rabbit
Target Name: RHOT1
Sample Tissue: Human A549 Whole Cell
Antibody Dilution: 1ug/ml
Rabbit Anti-RHOT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3207541

Western Blot (WB) of anti-RHOT1 antibody

Host: Rabbit
Target Name: RHOT1
Sample Tissue: Human HT1080 Whole Cell
Antibody Dilution: 1ug/ml
Rabbit Anti-RHOT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3207541

Western Blot (WB) of anti-RHOT1 antibody

Host: Rabbit
Target Name: RHOT1
Sample Type: Fetal Lung lysates
Antibody Dilution: 1.0ug/ml
Rabbit Anti-RHOT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3207541

Western Blot (WB) of anti-RHOT1 antibody

mouse brain and human neuroblastoma
Rabbit Anti-RHOT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3207541

Western Blot (WB) of anti-RHOT1 antibody

WB Suggested Anti-RHOT1 antibody Titration: 1 ug/mL
Sample Type: Human A549
Rabbit Anti-RHOT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3207541

Other Notes

Small volumes of anti-RHOT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RHOT1 purchase

MBS3207541 is an antibody targeting RHOT1. Also known as RHOT1 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RHOT1. It may not necessarily be applicable to this product.

NCBI GI #
75750470
NCBI GeneID
55288
NCBI Accession #
NP_060777
UniProt Primary Accession #
Q8IXI2
UniProt Related Accession #
Q8IXI2
GenBank Nucleotide #
NM_018307
NCBI Official Full Name
mitochondrial Rho GTPase 1 isoform 3
NCBI Official Synonym Full Names
ras homolog family member T1
NCBI Official Symbol
RHOT1
NCBI Official Synonym Symbols
ARHT1; MIRO1; MIRO-1
UniProt Protein Name
Mitochondrial Rho GTPase 1
UniProt Synonym Protein Names
Rac-GTP-binding protein-like protein; Ras homolog gene family member T1
UniProt Gene Name
RHOT1
UniProt Synonym Gene Names
ARHT1; MIRO-1; hMiro-1
UniProt Entry Name
MIRO1_HUMAN
NCBI Protein Information
mitochondrial Rho GTPase 1

UniProt Comments for RHOT1

RHOT1: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Belongs to the mitochondrial Rho GTPase family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; Membrane protein, integral; Hydrolase; Mitochondrial; G protein, monomeric, Rho; EC 3.6.5.-; G protein

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: membrane; plasma membrane; cytosol; integral to mitochondrial outer membrane

Molecular Function: GTPase activity; protein binding; GTP binding; calcium ion binding

Biological Process: regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; cellular homeostasis; mitochondrion transport along microtubule
Show more

Research Articles on RHOT1

1. The findings identify for the first time peroxisome-localized Miro1 variants as adapter proteins that link peroxisomes to the microtubule-dependent transport complexes including TRAK2 in the intracellular translocation of peroxisomes in mammalian cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000