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CHST1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3207783

This is a rabbit polyclonal antibody against CHST1. It was validated on Western Blot using a cell lysate as a positive control.Target Description: CHST1 catalyzes the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. It has a… More details

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Product Overview

Online Datasheet in PDF
Product Name
CHST1, Polyclonal Antibody
Full Product Name
CHST1 antibody - middle region
Product Gene Name
anti-CHST1 antibody
Product Synonym Gene Name
C6ST; KS6ST; KSGal6ST; KSST; GST-1
Immunogen Sequence
Synthetic peptide located within the following region: YRLWRLWYGT GRKPYNLDVT QLTTVCEDFS NSVSTGLMRP PWLKGKYMLV
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
CHST1 peptide (MBS3232749) is used for blocking the activity of CHST1 antibody (MBS3207783)
Molecular Weight
47kDa
OMIM
603797
3D Structure
ModBase 3D Structure for O43916
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 92%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human CHST1

Product Categories/Family for anti-CHST1 antibody

Polyclonal; Signal Proteins; Membrane Protein; Drugs and Drug Metabolism
Applications Tested/Suitable for anti-CHST1 antibody
Western Blot (WB)

Western Blot (WB) of anti-CHST1 antibody

Host: Rabbit
Target Name: CHST1
Sample Type: Human Fetal Heart
Antibody Dilution: 1.0ug/ml
Rabbit Anti-CHST1 Polyclonal Antibody – Cat# MBS3207783

Western Blot (WB) of anti-CHST1 antibody

Host: Rabbit
Target Name: CHST1
Sample Type: Human Fetal Lung
Antibody Dilution: 1.0ug/ml
Rabbit Anti-CHST1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3207783

Western Blot (WB) of anti-CHST1 antibody

WB Suggested Anti-CHST1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human kidney
Rabbit Anti-CHST1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3207783

Other Notes

Small volumes of anti-CHST1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHST1 purchase

MBS3207783 is an antibody targeting CHST1. Also known as CHST1 antibody - middle region. Reactive in Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHST1. It may not necessarily be applicable to this product.

NCBI GI #
4502841
NCBI GeneID
8534
NCBI Accession #
NP_003645
UniProt Primary Accession #
O43916
UniProt Related Accession #
O43916
GenBank Nucleotide #
NM_003654
NCBI Official Full Name
carbohydrate sulfotransferase 1
NCBI Official Synonym Full Names
carbohydrate sulfotransferase 1
NCBI Official Symbol
CHST1
NCBI Official Synonym Symbols
C6ST; KSST; GST-1; KS6ST; KSGal6ST
UniProt Protein Name
Carbohydrate sulfotransferase 1
UniProt Synonym Protein Names
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase
Protein Family
Carbohydrate sulfotransferase
UniProt Gene Name
CHST1
UniProt Synonym Gene Names
GST-1; KS6ST; KSGal6ST; KSST
UniProt Entry Name
CHST1_HUMAN
NCBI Protein Information
carbohydrate sulfotransferase 1

NCBI Summary for CHST1

This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]

UniProt Comments for CHST1

CHST1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N- acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: Membrane protein, integral; Transferase; EC 2.8.2.21; Glycan Metabolism - keratan sulfate biosynthesis

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: sulfotransferase activity; keratan sulfotransferase activity

Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; galactose metabolic process; polysaccharide metabolic process; pathogenesis; inflammatory response
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Research Articles on CHST1

1. found endogenous expression of the long form of the enzyme in human tissue, predominantly in the trans-Golgi network of endothelial cells that form human high endothelial venules.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CHST1 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
 Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 Keratan Sulfate Biosynthesis Pathway antibodies  Keratan Sulfate Biosynthesis Pathway Diagram
 Keratan Sulfate/keratin Metabolism Pathway antibodies  Keratan Sulfate/keratin Metabolism Pathway Diagram
 MPS I - Hurler Syndrome Pathway antibodies  MPS I - Hurler Syndrome Pathway Diagram
 MPS II - Hunter Syndrome Pathway antibodies  MPS II - Hunter Syndrome Pathway Diagram
 MPS IIIA - Sanfilippo Syndrome A Pathway antibodies  MPS IIIA - Sanfilippo Syndrome A Pathway Diagram
 MPS IIIB - Sanfilippo Syndrome B Pathway antibodies  MPS IIIB - Sanfilippo Syndrome B Pathway Diagram

Diseases associated with anti-CHST1 antibody

 Disease Name  Pubmed Publications
 Drug Toxicity Antibodies  >2 publications with CHST1 and Drug Toxicity
 Inflammation Antibodies  >2 publications with CHST1 and Inflammation

Organs/Tissues associated with anti-CHST1 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1 publications with CHST1 and Eye
 Lung Antibodies  >1 publications with CHST1 and Lung
 Brain Antibodies  >1 publications with CHST1 and Brain
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