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GUCY1B1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3207995

This is a rabbit polyclonal antibody against GUCY1B3. It was validated on Western Blot using a cell lysate as a positive control.Target Description: This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of… More details

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Product Overview

Online Datasheet in PDF
Product Name
GUCY1B1, Polyclonal Antibody
Full Product Name
GUCY1B1 Antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
GUCB3; GC-SB3; GUC1B3; GUCSB3; GUCY1B3; GC-S-beta-1
Immunogen Sequence
Synthetic peptide located within the following region: LIEEKESKEE DFYEDLDRFE ENGTQESRIS PYTFCKAFPF HIIFDRDLVV
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
GUCY1B1 peptide (MBS3232958) is used for blocking the activity of GUCY1B1 antibody (MBS3207995)
Molecular Weight
70kDa
OMIM
139397
3D Structure
ModBase 3D Structure for Q02153
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human GUCY1B3
Applications Tested/Suitable for anti-GUCY1B1 antibody
Western Blot (WB)

Western Blot (WB) of anti-GUCY1B1 antibody

Host: Rabbit
Target Name: GCYB1
Sample Type: Fetal Brain lysates
Antibody Dilution: 0.5ug/ml
Rabbit Anti-GUCY1B1 Polyclonal Antibody – Cat# MBS3207995

Western Blot (WB) of anti-GUCY1B1 antibody

WB Suggested Anti-GUCY1B3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: 293T cell lysateGUCY1B3 is supported by BioGPS gene expression data to be expressed in HEK293T
Rabbit Anti-GUCY1B1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3207995

Other Notes

Small volumes of anti-GUCY1B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GUCY1B1 purchase

MBS3207995 is an antibody targeting GUCY1B1. Also known as GUCY1B1 Antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GUCY1B1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
guanylate cyclase soluble subunit beta-1 isoform 2
NCBI Official Synonym Full Names
guanylate cyclase 1 soluble subunit beta 1
NCBI Official Symbol
GUCY1B1
NCBI Official Synonym Symbols
GUCB3; GC-SB3; GUC1B3; GUCSB3; GUCY1B3; GC-S-beta-1
UniProt Protein Name
Guanylate cyclase soluble subunit beta-1
UniProt Synonym Protein Names
Guanylate cyclase soluble subunit beta-3; GCS-beta-3; Soluble guanylate cyclase small subunit
UniProt Gene Name
GUCY1B3
UniProt Synonym Gene Names
GUC1B3; GUCSB3; GUCY1B1; GCS-beta-1; GCS-beta-3
UniProt Entry Name
GCYB1_HUMAN
NCBI Protein Information
guanylate cyclase soluble subunit beta-1

NCBI Summary for GUCY1B1

This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
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UniProt Comments for GUCY1B1

GUCY1B3: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Heterodimer of an alpha and a beta chain. 2 isoforms of the human protein are produced by alternative splicing

Protein type: EC 4.6.1.2; Guanylyl cyclase; Nucleotide Metabolism - purine; Receptor, misc.; Lyase

Chromosomal Location of Human Ortholog: 4q31.3-q33

Cellular Component: guanylate cyclase complex, soluble; intracellular membrane-bound organelle; cytoplasm

Molecular Function: guanylate cyclase activity; GTP binding; protein heterodimerization activity; metal ion binding; heme binding; Hsp90 protein binding; receptor activity

Biological Process: cGMP biosynthetic process; blood circulation; blood coagulation; nitric oxide mediated signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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