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NMT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3208148

This is a rabbit polyclonal antibody against NMT1. It was validated on Western Blot using a cell lysate as a positive control.Target Description: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the… More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NMT1, Polyclonal Antibody
Full Product Name
NMT1 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
NMT
Immunogen Sequence
Synthetic peptide located within the following region: TMEEASKRSY QFWDTQPVPK LGEVVNTHGP VEPDKDNIRQ EPYTLPQGFT
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
NMT1 peptide (MBS3233111) is used for blocking the activity of NMT1 antibody (MBS3208148)
Molecular Weight
57kDa
OMIM
160993
3D Structure
ModBase 3D Structure for P30419
Homology
Cow: 100%; Dog: 93%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human NMT1
Applications Tested/Suitable for anti-NMT1 antibody
Western Blot (WB)

Western Blot (WB) of anti-NMT1 antibody

WB Suggested Anti-NMT1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysateNMT1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
Rabbit Anti-NMT1 Polyclonal Antibody – Cat# MBS3208148

Other Notes

Small volumes of anti-NMT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NMT1 purchase

MBS3208148 is an antibody targeting NMT1. Also known as NMT1 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NMT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glycylpeptide N-tetradecanoyltransferase 1
NCBI Official Synonym Full Names
N-myristoyltransferase 1
NCBI Official Symbol
NMT1
NCBI Official Synonym Symbols
NMT
UniProt Protein Name
Glycylpeptide N-tetradecanoyltransferase 1
UniProt Synonym Protein Names
Myristoyl-CoA:protein N-myristoyltransferase 1; NMT 1; Type I N-myristoyltransferase; Peptide N-myristoyltransferase 1
UniProt Gene Name
NMT1
UniProt Synonym Gene Names
NMT; NMT 1; Type I N-myristoyltransferase
UniProt Entry Name
NMT1_HUMAN
NCBI Protein Information
glycylpeptide N-tetradecanoyltransferase 1

NCBI Summary for NMT1

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008]
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UniProt Comments for NMT1

NMT1: Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Belongs to the NMT family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.97; Motility/polarity/chemotaxis; Transferase

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: actin cytoskeleton; cell junction; cytoplasm; cytosol; extrinsic to membrane; plasma membrane

Molecular Function: glycylpeptide N-tetradecanoyltransferase activity; myristoyltransferase activity

Biological Process: N-terminal peptidyl-glycine N-myristoylation; N-terminal protein myristoylation; regulation of rhodopsin mediated signaling
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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