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PDIA4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3208240

This is a rabbit polyclonal antibody against PDIA4. It was validated on Western Blot using a cell lysate as a positive control.Target Description: The specific function of PDIA4 is not yet known. More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDIA4, Polyclonal Antibody
Full Product Name
PDIA4 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
ERP70; ERP72; ERp-72
Immunogen Sequence
Synthetic peptide located within the following region: ENAIEDEEEE EEEDDDEEED DLEVKEENGV LVLNDANFDN FVADKDTVLL
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
PDIA4 peptide (MBS3233203) is used for blocking the activity of PDIA4 antibody (MBS3208240)
Molecular Weight
73kDa
3D Structure
ModBase 3D Structure for P13667
Homology
Cow: 86%; Dog: 100%; Human: 100%; Mouse: 87%; Pig: 100%; Rabbit: 92%; Rat: 92%; Zebrafish: 92%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human PDIA4
Applications Tested/Suitable for anti-PDIA4 antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-PDIA4 antibody

Immunohistochemistry with Rat Brain lysate tissue
Rabbit Anti-PDIA4 Polyclonal Antibody – Cat# MBS3208240

Western Blot (WB) of anti-PDIA4 antibody

rat brain
Rabbit Anti-PDIA4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3208240

Western Blot (WB) of anti-PDIA4 antibody

WB Suggested Anti-PDIA4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Liver
Rabbit Anti-PDIA4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3208240

Other Notes

Small volumes of anti-PDIA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDIA4 purchase

MBS3208240 is an antibody targeting PDIA4. Also known as PDIA4 antibody - N-terminal region. Reactive in Cow, Dog, Human, Mouse, Pig, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PDIA4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein disulfide-isomerase A4
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 4
NCBI Official Symbol
PDIA4
NCBI Official Synonym Symbols
ERP70; ERP72; ERp-72
UniProt Protein Name
Protein disulfide-isomerase A4
UniProt Synonym Protein Names
Endoplasmic reticulum resident protein 70; ER protein 70; ERp70; Endoplasmic reticulum resident protein 72; ER protein 72; ERp-72; ERp72
UniProt Gene Name
PDIA4
UniProt Synonym Gene Names
ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72
UniProt Entry Name
PDIA4_HUMAN
NCBI Protein Information
protein disulfide-isomerase A4

NCBI Summary for PDIA4

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. [provided by RefSeq, Dec 2016]
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UniProt Comments for PDIA4

PDIA4: Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the protein disulfide isomerase family.

Protein type: EC 5.3.4.1; Isomerase; Chaperone; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 7q35

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: protein folding; cell redox homeostasis; protein secretion
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Research Articles on PDIA4

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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