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GOLGA5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3208248

This is a rabbit polyclonal antibody against GOLGA5. It was validated on Western Blot using a cell lysate as a positive control.Target Description: The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GOLGA5, Polyclonal Antibody
Full Product Name
GOLGA5 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
GOLIM5; RFG5; ret-II
Immunogen Sequence
Synthetic peptide located within the following region: FVRRKKSEPD DELLFDFLNS SQKEPTGRVE IRKEKGKTPV FQSSQTSSVS
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
GOLGA5 peptide (MBS3233211) is used for blocking the activity of GOLGA5 antibody (MBS3208248)
Molecular Weight
83kDa
OMIM
phenotype 606918
3D Structure
ModBase 3D Structure for Q8TBA6
Homology
Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 86%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human GOLGA5
Applications Tested/Suitable for anti-GOLGA5 antibody
Western Blot (WB)

Western Blot (WB) of anti-GOLGA5 antibody

Host: Rabbit
Target Name: GOLGA5
Sample Type: Human 293T
Antibody Dilution: 1.0ug/mlGOLGA5 is supported by BioGPS gene expression data to be expressed in HEK293T
Rabbit Anti-GOLGA5 Polyclonal Antibody – Cat# MBS3208248

Western Blot (WB) of anti-GOLGA5 antibody

WB Suggested Anti-GOLGA5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysateGOLGA5 is supported by BioGPS gene expression data to be expressed in 721_B
Rabbit Anti-GOLGA5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3208248

Other Notes

Small volumes of anti-GOLGA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOLGA5 purchase

MBS3208248 is an antibody targeting GOLGA5. Also known as GOLGA5 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GOLGA5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
golgin subfamily A member 5
NCBI Official Synonym Full Names
golgin A5
NCBI Official Symbol
GOLGA5
NCBI Official Synonym Symbols
RFG5; GOLIM5; ret-II
UniProt Protein Name
Golgin subfamily A member 5
UniProt Synonym Protein Names
Cell proliferation-inducing gene 31 protein; Golgin-84; Protein Ret-II; RET-fused gene 5 protein
Protein Family
UniProt Gene Name
GOLGA5
UniProt Synonym Gene Names
RETII; RFG5
UniProt Entry Name
GOGA5_HUMAN
NCBI Protein Information
golgin subfamily A member 5

NCBI Summary for GOLGA5

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. [provided by RefSeq, Jul 2013]
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UniProt Comments for GOLGA5

GOLGA5: a member of the golgin family of proteins, whose members localize to the Golgi. Involved in regulating the architecture of the Golgi apparatus. A coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues. Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase. Two splice variant isoforms have been described.

Protein type: Membrane protein, integral; Cytoskeletal

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: Golgi membrane; Golgi apparatus; Golgi cisterna; cis-Golgi network; membrane; integral to membrane

Molecular Function: protein homodimerization activity; Rab GTPase binding

Biological Process: Golgi organization and biogenesis; Golgi vesicle transport

Disease: Thyroid Carcinoma, Papillary
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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