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ATP6V0A1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3208252

This is a rabbit polyclonal antibody against ATP6V0A1. It was validated on Western Blot using a cell lysate as a positive control.Target Description: ATP6V0A1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of… More details

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Product Overview

Online Datasheet in PDF
Product Name
ATP6V0A1, Polyclonal Antibody
Full Product Name
ATP6V0A1 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
ATP6N1; ATP6N1A; DKFZp781J1951; Stv1; VPP1; Vph1; a1
Immunogen Sequence
Synthetic peptide located within the following region: RDLNPDVNVF QRKFVNEVRR CEEMDRKLRF VEKEIRKANI PIMDTGENPE
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
Molecular Weight
96kDa
OMIM
192130
3D Structure
ModBase 3D Structure for Q93050
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human ATP6V0A1
Applications Tested/Suitable for anti-ATP6V0A1 antibody
Western Blot (WB)

Western Blot (WB) of anti-ATP6V0A1 antibody

WB Suggested Anti-ATP6V0A1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Hela cell lysate
Rabbit Anti-ATP6V0A1 Polyclonal Antibody – Cat# MBS3208252

Other Notes

Small volumes of anti-ATP6V0A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATP6V0A1 purchase

MBS3208252 is an antibody targeting ATP6V0A1. Also known as ATP6V0A1 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATP6V0A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
V-type proton ATPase 116 kDa subunit a isoform 1 isoform c
NCBI Official Synonym Full Names
ATPase H+ transporting V0 subunit a1
NCBI Official Symbol
ATP6V0A1
NCBI Official Synonym Symbols
a1; Stv1; VPP1; Vph1; ATP6N1; ATP6N1A
UniProt Protein Name
V-type proton ATPase 116 kDa subunit a isoform 1
UniProt Synonym Protein Names
Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit; Vacuolar adenosine triphosphatase subunit Ac116; Vacuolar proton pump subunit 1; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1
Protein Family
UniProt Gene Name
ATP6V0A1
UniProt Synonym Gene Names
ATP6N1; ATP6N1A; VPP1; V-ATPase 116 kDa isoform a1
UniProt Entry Name
VPP1_HUMAN
NCBI Protein Information
V-type proton ATPase 116 kDa subunit a isoform 1; V-type proton ATPase 116 kDa subunit a

NCBI Summary for ATP6V0A1

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for ATP6V0A1

Function: Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle

By similarity.

Subunit structure: The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''.

Subcellular location: Cytoplasmic vesicle membrane; Multi-pass membrane protein. Melanosome. Note: Coated vesicle. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.7 Ref.8

Sequence similarities: Belongs to the V-ATPase 116 kDa subunit family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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