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ICMT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3208378

This is a rabbit polyclonal antibody against ICMT. It was validated on Western Blot using a cell lysate as a positive control.Target Description: ICMT is the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in… More details

$490 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
ICMT, Polyclonal Antibody
Full Product Name
ICMT antibody - middle region
Product Gene Name
Product Synonym Gene Name
HSTE14; MGC39955; MST098; MSTP098; PCCMT; PCMT; PPMT
Immunogen Sequence
Synthetic peptide located within the following region: GSNFNHVVQN EKSDTHTLVT SGVYAWFRHP SYVGWFYWSI GTQVMLCNPI
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
ICMT peptide (MBS3233340) is used for blocking the activity of ICMT antibody (MBS3208378)
Molecular Weight
32kDa
OMIM
AF064084 mRNA
3D Structure
ModBase 3D Structure for O60725
Homology
Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 79%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human ICMT
Applications Tested/Suitable for anti-ICMT antibody
Western Blot (WB)

Western Blot (WB) of anti-ICMT antibody

WB Suggested Anti-ICMT Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human heart
Rabbit Anti-ICMT Polyclonal Antibody – Cat# MBS3208378

Other Notes

Small volumes of anti-ICMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ICMT purchase

MBS3208378 is an antibody targeting ICMT. Also known as ICMT antibody - middle region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ICMT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein-S-isoprenylcysteine O-methyltransferase
NCBI Official Synonym Full Names
isoprenylcysteine carboxyl methyltransferase
NCBI Official Symbol
ICMT
NCBI Official Synonym Symbols
PCMT; PPMT; PCCMT; HSTE14; MST098; MSTP098
UniProt Protein Name
Protein-S-isoprenylcysteine O-methyltransferase
UniProt Synonym Protein Names
Isoprenylcysteine carboxylmethyltransferase; Prenylated protein carboxyl methyltransferase; PPMT; Prenylcysteine carboxyl methyltransferase; pcCMT
UniProt Gene Name
ICMT
UniProt Synonym Gene Names
PCCMT; PPMT; pcCMT
NCBI Protein Information
protein-S-isoprenylcysteine O-methyltransferase

NCBI Summary for ICMT

This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for ICMT

ICMT: Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues. Belongs to the isoprenylcysteine O-methyltransferase family.

Protein type: EC 2.1.1.100; Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass; Methyltransferase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.31

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral component of membrane; membrane

Molecular Function: protein C-terminal carboxyl O-methyltransferase activity; protein-S-isoprenylcysteine O-methyltransferase activity

Biological Process: C-terminal protein methylation; cellular protein modification process; post-translational protein modification; protein targeting to membrane
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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