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STK3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3209127

This is a rabbit polyclonal antibody against STK3. It was validated on Western Blot and immunohistochemistryTarget Description: Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or… More details

$440 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
STK3, Polyclonal Antibody
Full Product Name
STK3 antibody - C-terminal region
Product Gene Name
Product Synonym Gene Name
FLJ90748; KRS1; MST2
Immunogen Sequence
Synthetic peptide located within the following region: IEHNSTMLES DLGTMVINSE DEEEEDGTMK RNATSPQVQR PSFMDYFDKQ
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Protein A purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
STK3 peptide (MBS3234090) is used for blocking the activity of STK3 antibody (MBS3209127)
Molecular Weight
56kDa
OMIM
U26424 mRNA
3D Structure
ModBase 3D Structure for Q13188
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 77%
Immunogen
The immunogen is a synthetic peptide directed towards the C terminal region of human STK3
Applications Tested/Suitable for anti-STK3 antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-STK3 antibody

Rabbit Anti-STK3 Antibody
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Rabbit Anti-STK3 Polyclonal Antibody – Cat# MBS3209127

Western Blot (WB) of anti-STK3 antibody

Host: Mouse
Target Name: STK3
Sample Tissue: Mouse Heart
Antibody Dilution: 1ug/ml
Rabbit Anti-STK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3209127

Western Blot (WB) of anti-STK3 antibody

Host: Rabbit
Target Name: STK3
Sample Tissue: Human Lung Tumor
Antibody Dilution: 1.0ug/ml
Rabbit Anti-STK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3209127

Western Blot (WB) of anti-STK3 antibody

Lanes:
Lane1: 100ug uninduced RPE-1 lysate
Lane2: 100ug uninduced RPE-1 lysate
Lane3: 100ug STK3 induced RPE-1 lysate
Lane4: 100ug STK3 induced RPE-1 lysate
Primary Antibody Dilution:
1:2000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
STK3
Submitted by:
Anonymous
Rabbit Anti-STK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3209127

Western Blot (WB) of anti-STK3 antibody

Lanes:
Lane1: 100ug untransfected COS-7 lysate
Lane2: 100ug mock transfected Cos-7 lysate
Lane3: 100ug STK3 transfected Cos-7 lysate
Lane4: 50 ug STK3 transfected Cos-7 lysate
Lane5: 25 ug STK3 transfected Cos-7 lysate
Primary Antibody Dilution:
1:2000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
STK3
Submitted by:
Anonymous
Rabbit Anti-STK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3209127

Western Blot (WB) of anti-STK3 antibody

WB Suggested Anti-STK3 Antibody Titration: 5.0ug/ml
Positive Control: HepG2 cell lysate
Rabbit Anti-STK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3209127

Other Notes

Small volumes of anti-STK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for STK3 purchase

MBS3209127 is an antibody targeting STK3. Also known as STK3 antibody - C-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for STK3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase 3 isoform 1
NCBI Official Synonym Full Names
serine/threonine kinase 3
NCBI Official Symbol
STK3
NCBI Official Synonym Symbols
KRS1; MST2
UniProt Protein Name
Serine/threonine-protein kinase 3
UniProt Synonym Protein Names
Mammalian STE20-like protein kinase 2; MST-2
UniProt Gene Name
STK3
UniProt Synonym Gene Names
KRS1; MST2; MST-2; MST2/N; MST2/C
NCBI Protein Information
serine/threonine-protein kinase 3

NCBI Summary for STK3

This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
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UniProt Comments for STK3

MST2: a protein kinase of the STE20 family. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Following cleavage by caspase-3, undergoes irreversible autophosphorylation. Full-length is mainly unphosphorylated in the rat thymus and cultured cells, whereas caspase-3-cleaved MST2 in apoptotic cells is highly phosphorylated.

Protein type: Autophagy; EC 2.7.11.1; Kinase, protein; MST subfamily; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: cytoplasm; cytosol; protein complex

Molecular Function: ATP binding; magnesium ion binding; protein binding; protein dimerization activity; protein kinase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of protein binding; positive regulation of transcription factor activity; protein amino acid phosphorylation; protein stabilization; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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