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CYP3A43 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3209612

This is a rabbit polyclonal antibody against CYP3A43. It was validated on Western Blot using a cell lysate as a positive control.Target Description: CYP3A43 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are… More details

$490 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CYP3A43, Polyclonal Antibody
Full Product Name
CYP3A43 antibody - middle region
Product Gene Name
Product Synonym Gene Name
MGC119315; MGC119316
Immunogen Sequence
Synthetic peptide located within the following region: ERMKESRLKD KQKHRVDFFQ QMIDSQNSKE TKSHKALSDL ELVAQSIIII
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Human, Rabbit, Rat, Sheep
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
CYP3A43 peptide (MBS3234568) is used for blocking the activity of CYP3A43 antibody (MBS3209612)
Molecular Weight
58kDa
OMIM
606534
3D Structure
ModBase 3D Structure for Q9HB55
Homology
Cow: 79%; Dog: 79%; Guinea Pig: 79%; Human: 100%; Rabbit: 79%; Rat: 79%; Sheep: 79%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human CYP3A43
Applications Tested/Suitable for anti-CYP3A43 antibody
Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry (IHC) of anti-CYP3A43 antibody

CYP3A43 antibody - middle region

Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue

Observed Staining: Cytoplasm in Kupffer cells of liver

Primary Antibody Concentration: 1:600

Secondary Antibody: Donkey anti-Rabbit-Cy3

Secondary Antibody Concentration: 1:200

Magnification: 20X

Exposure Time: 0.5 - 2.0 sec
Rabbit Anti-CYP3A43 Polyclonal Antibody – Cat# MBS3209612

Western Blot (WB) of anti-CYP3A43 antibody

WB Suggested Anti-CYP3A43 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human brain
Rabbit Anti-CYP3A43 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3209612

Other Notes

Small volumes of anti-CYP3A43 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYP3A43 purchase

MBS3209612 is an antibody targeting CYP3A43. Also known as CYP3A43 antibody - middle region. Reactive in Cow, Dog, Guinea Pig, Human, Rabbit, Rat, Sheep. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CYP3A43. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cytochrome P450 3A43 isoform 1
NCBI Official Synonym Full Names
cytochrome P450 family 3 subfamily A member 43
NCBI Official Symbol
CYP3A43
UniProt Protein Name
Cytochrome P450 3A43
Protein Family
UniProt Gene Name
CYP3A43
UniProt Entry Name
CP343_HUMAN
NCBI Protein Information
cytochrome P450 3A43

NCBI Summary for CYP3A43

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein has a low level of testosterone hydroxylase activity, and may play a role in aging mechanisms and cancer progression. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
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UniProt Comments for CYP3A43

CYP3A43: Exhibits low testosterone 6-beta-hydroxylase activity. Belongs to the cytochrome P450 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.14.1; Oxidoreductase; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: endoplasmic reticulum membrane

Molecular Function: iron ion binding; heme binding; monooxygenase activity

Biological Process: xenobiotic metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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