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CRAT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3211368

This is a rabbit polyclonal antibody against CRAT. It was validated on Western Blot using a cell lysate as a positive control.Target Description: Carnitine acetyltransferase (CRAT) is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and… More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CRAT, Polyclonal Antibody
Full Product Name
CRAT antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
RP11-247A12.5; CAT1
Immunogen Sequence
Synthetic peptide located within the following region: MKASSRFKAH QDALPRLPVP PLQQSLDHYL KALQPIVSEE EWAHTKQLVD
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
CRAT peptide (MBS3236319) is used for blocking the activity of CRAT antibody (MBS3211368)
Molecular Weight
51kDa
OMIM
600184
3D Structure
ModBase 3D Structure for P43155
Homology
Cow: 92%; Dog: 92%; Guinea Pig: 91%; Horse: 85%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 92%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human CRAT
Applications Tested/Suitable for anti-CRAT antibody
Western Blot (WB)

Immunohistochemistry (IHC) of anti-CRAT antibody

Rabbit Anti-CRAT antibody
Formalin Fixed Paraffin Embedded Tissue: Human Kidney
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec
Rabbit Anti-CRAT Polyclonal Antibody – Cat# MBS3211368

Immunohistochemistry (IHC) of anti-CRAT antibody

Rabbit Anti-CRAT antibody
Formalin Fixed Paraffin Embedded Tissue: Human Liver
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec
Rabbit Anti-CRAT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3211368

Western Blot (WB) of anti-CRAT antibody

Host: Rabbit
Target Name: CRAT
Sample Type: Human HepG2, Liver, Testis
Antibody Dilution: 2.0 ug/ml
Rabbit Anti-CRAT Polyclonal Antibody, Western Blot (WB) – Cat# MBS3211368

Western Blot (WB) of anti-CRAT antibody

WB Suggested Anti-CRAT Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate
Rabbit Anti-CRAT Polyclonal Antibody, Western Blot (WB) – Cat# MBS3211368

Other Notes

Small volumes of anti-CRAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CRAT purchase

MBS3211368 is an antibody targeting CRAT. Also known as CRAT antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CRAT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carnitine acetyltransferase, isoform CRA_a, partial
NCBI Official Synonym Full Names
carnitine O-acetyltransferase
NCBI Official Symbol
CRAT
NCBI Official Synonym Symbols
CAT; CAT1; NBIA8
UniProt Protein Name
Carnitine O-acetyltransferase
UniProt Synonym Protein Names
Carnitine acetyltransferase; CAT; CrAT
UniProt Gene Name
CRAT
UniProt Synonym Gene Names
CAT1; Carnitine acetylase; CAT; CrAT
UniProt Entry Name
CACP_HUMAN
NCBI Protein Information
carnitine O-acetyltransferase

NCBI Summary for CRAT

This gene encodes carnitine O-acetyltransferase, a member of the carnitine acyltransferase family and a key metabolic pathway enzyme which plays an important role in energy homeostasis and fat metabolism. This enzyme catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acyl-CoA/CoA. It is found in both the mitochondria and the peroxisome. Alternative splicing results in transcript variants encoding different isoforms that may localize to different subcellular compartments. [provided by RefSeq, Oct 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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