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HMGCLL1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3212675

This is a rabbit polyclonal antibody against HMGCLL1. It was validated on Western Blot using a cell lysate as a positive control.Target Description: HMGCLL1 is involved in the catabolism of branched amino acids such as leucine. More details

$490 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HMGCLL1, Polyclonal Antibody
Full Product Name
HMGCLL1 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
DKFZP434G1411; bA418P12.1
Immunogen Sequence
Synthetic peptide located within the following region: MGNVPSAVKH CLSYQQLLRE HLWIGDSVAG ALDPAQETSQ LSGLPEFVKI
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Horse, Human, Mouse, Rabbit, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
HMGCLL1 peptide (MBS3237622) is used for blocking the activity of HMGCLL1 antibody (MBS3212675)
Molecular Weight
36kDa
3D Structure
ModBase 3D Structure for Q8TB92
Homology
Cow: 92%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 92%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human HMGCLL1

Product Categories/Family for anti-HMGCLL1 antibody

Applications Tested/Suitable for anti-HMGCLL1 antibody
Western Blot (WB)

Western Blot (WB) of anti-HMGCLL1 antibody

Host: Rabbit
Target Name: HMGCLL1
Sample Tissue: Human DLD1 Whole Cell
Antibody Dilution: 1ug/ml
Rabbit Anti-HMGCLL1 Polyclonal Antibody – Cat# MBS3212675

Western Blot (WB) of anti-HMGCLL1 antibody

Host: Rabbit
Target Name: HMGCLL1
Sample Tissue: Human THP-1 Whole Cell
Antibody Dilution: 1ug/ml
Rabbit Anti-HMGCLL1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3212675

Western Blot (WB) of anti-HMGCLL1 antibody

WB Suggested Anti-HMGCLL1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: OVCAR-3 cell lysate
Rabbit Anti-HMGCLL1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3212675

Other Notes

Small volumes of anti-HMGCLL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HMGCLL1 purchase

MBS3212675 is an antibody targeting HMGCLL1. Also known as HMGCLL1 antibody - N-terminal region. Reactive in Cow, Horse, Human, Mouse, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HMGCLL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic isoform b
NCBI Official Synonym Full Names
3-hydroxymethyl-3-methylglutaryl-CoA lyase like 1
NCBI Official Symbol
HMGCLL1
NCBI Official Synonym Symbols
er-cHL; bA418P12.1
UniProt Protein Name
3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic
UniProt Synonym Protein Names
3-hydroxymethyl-3-methylglutaryl-CoA lyase-like protein 1; Endoplasmic reticulum 3-hydroxymethyl-3-methylglutaryl-CoA lyase; er-cHL
UniProt Gene Name
HMGCLL1
UniProt Synonym Gene Names
er-cHL
NCBI Protein Information
3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic

UniProt Comments for HMGCLL1

HMGCLL1: Non-mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy- 3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis, the products of which support energy production in nonhepatic animal tissues. Belongs to the HMG-CoA lyase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.1.3.4; Lyase

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm

Molecular Function: hydroxymethylglutaryl-CoA lyase activity; metal ion binding

Biological Process: ketone body biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HMGCLL1 antibody

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