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C22orf28 (RTCB) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3212971

MBS3212971 is an antibody targeting RTCB. Also known as C22orf28 antibody - N-terminal region. Reactive in Tested Species Reactivity: HumanPredicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit. Target… More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
C22orf28 (RTCB), Polyclonal Antibody
Full Product Name
C22orf28 antibody - N-terminal region
Product Gene Name
anti-RTCB antibody
Product Synonym Gene Name
DJ149A16.6; HSPC117; RP1-149A16.6; C22orf28
Product Synonym Names
FAAP, HSPC117, C22orf28, DJ149A16.6
Immunogen Sequence
Synthetic peptide located within the following region: PEAVVSPGGV GFDINCGVRL LRTNLDESDV QPVKEQLAQA MFDHIPVGVG
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Concentration
0.5 mg/mL (lot specific)
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
C22orf28 peptide (MBS3237916) is used for blocking the activity of C22orf28 antibody (MBS3212971)
Molecular Weight
55kDa
OMIM
613901
3D Structure
ModBase 3D Structure for Q9Y3I0
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human C22orf28
Protein Size (# AA)
505 amino acids
Protein Interactions
UBC; FUS; SUMO1; NEDD8; MDM2; BMI1; rev; HNRNPM; MRE11A; KRT18; ILF2; FLII; DHX9; DDX1; ABCF1; C14orf166; RPL26L1; IGF2BP3; POLR1C; LRRFIP1; EIF2B2; EIF2B3; YBX3; RPL27; RFC4; RFC2; QARS; PRKDC; NMT1; AICDA; FN1; CA9; FAM98B; C2orf49; CAND1; COPS5; CUL1
Blocking Peptide
For anti-RTCB (MBS3212971) antibody is MBS3237916
Protein Name
tRNA-splicing ligase RtcB homolog
Enhanced Validation
WB
Y
SPR
YCHAROS

Product Categories/Family for anti-RTCB antibody

Polyclonal; Various
Applications Tested/Suitable for anti-RTCB antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-RTCB antibody

Rabbit Anti-C22orf28 antibody
Formalin Fixed Paraffin Embedded Tissue: Human Colon
Primary antibody Concentration: 10 ug/ml
Rabbit Anti-RTCB Polyclonal Antibody – Cat# MBS3212971

Immunohistochemistry (IHC) of anti-RTCB antibody

Rabbit Anti-C22orf28 antibody
Formalin Fixed Paraffin Embedded Tissue: Human Kidney
Primary antibody Concentration: 10 ug/ml
Rabbit Anti-RTCB Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3212971

Western Blot (WB) of anti-RTCB antibody

Host: Rabbit
Target Name: C22orf28
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml
Rabbit Anti-RTCB Polyclonal Antibody, Western Blot (WB) – Cat# MBS3212971

Western Blot (WB) of anti-RTCB antibody

Host: Rabbit
Target Name: C22orf28
Sample Type: Hela
Antibody Dilution: 1.0ug/mlRTCB is supported by BioGPS gene expression data to be expressed in HeLa
Rabbit Anti-RTCB Polyclonal Antibody, Western Blot (WB) – Cat# MBS3212971

Western Blot (WB) of anti-RTCB antibody

Host: Rabbit
Target Name: C22orf28
Sample Type: Human Fetal Lung
Antibody Dilution: 1.0ug/ml
Rabbit Anti-RTCB Polyclonal Antibody, Western Blot (WB) – Cat# MBS3212971

Western Blot (WB) of anti-RTCB antibody

Host: Rabbit
Target Name: C22orf28
Sample Type: Jurkat
Antibody Dilution: 1.0ug/mlRTCB is supported by BioGPS gene expression data to be expressed in Jurkat
Rabbit Anti-RTCB Polyclonal Antibody, Western Blot (WB) – Cat# MBS3212971

Western Blot (WB) of anti-RTCB antibody

WB Suggested Anti-C22orf28 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate
Rabbit Anti-RTCB Polyclonal Antibody, Western Blot (WB) – Cat# MBS3212971

Western Blot (WB) of anti-RTCB antibody

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Protein may be modified by phosphorylation.
Rabbit Anti-RTCB Polyclonal Antibody, Western Blot (WB) – Cat# MBS3212971

Immunofluorescence (IF) of anti-RTCB antibody

Rabbit Anti-RTCB Antibody
Catalog Number: MBS3212971
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3b
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec.
Rabbit Anti-RTCB Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS3212971

Other Notes

Small volumes of anti-RTCB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RTCB. It may not necessarily be applicable to this product.

NCBI GI #
7657015
NCBI GeneID
51493
NCBI Accession #
NP_055121
UniProt Primary Accession #
Q9Y3I0
UniProt Related Accession #
Q9Y3I0
GenBank Nucleotide #
NM_014306
NCBI Official Full Name
tRNA-splicing ligase RtcB homolog
NCBI Official Synonym Full Names
RNA 2',3'-cyclic phosphate and 5'-OH ligase
NCBI Official Symbol
RTCB
NCBI Official Synonym Symbols
FAAP; HSPC117; C22orf28; DJ149A16.6
UniProt Protein Name
tRNA-splicing ligase RtcB homolog
Protein Family
RNA-splicing ligase
UniProt Gene Name
RTCB
NCBI Protein Information
tRNA-splicing ligase RtcB homolog

UniProt Comments for RTCB

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Show more

Research Articles on RTCB

1. This paper presents a homology model of human RtcB (hRtcB). The structure was analyzed in terms of stereochemical quality, folding reliability, secondary structure, druggability of the active site binding pocket and its metal-binding microenvironment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RTCB antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 TRNA Processing Pathway antibodies  TRNA Processing Pathway Diagram
 TRNA Processing In The Nucleus Pathway antibodies  TRNA Processing In The Nucleus Pathway Diagram

Diseases associated with anti-RTCB antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with RTCB and Nervous System Diseases

Organs/Tissues associated with anti-RTCB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with RTCB and Blood
 Eye Antibodies  >1 publications with RTCB and Eye
 Brain Antibodies  >1 publications with RTCB and Brain
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