Rabbit Anti-NELFCD Polyclonal Antibody – Cat# MBS3213571

WB Suggested Anti-TH1L Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysateNELFCD is supported by BioGPS gene expression data to be expressed in Jurkat

NELFCD Antibody – Rabbit Polyclonal

Name: NELFCD Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS3213571
Price: 490 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3213571

This is a rabbit polyclonal antibody against TH1L. It was validated on Western BlotTarget Description: The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this… More details

$490 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TH1L (NELFCD), Polyclonal Antibody

Full Product Name

TH1L antibody - N-terminal region

Product Gene Name

anti-NELFCD antibody

Product Synonym Gene Name

HSPC130; NELF-C; NELF-D; TH1; TH1L

Immunogen Sequence

Synthetic peptide located within the following region: GEGEDDAEVQ QECLHKFSTR DYIMEPSIFN TLKRYFQAGG SPENVIQLLS

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Form/Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Purity/Purification

Affinity Purified

Preparation and Storage

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Antibody/Peptide Pairs

TH1L peptide (MBS3238513) is used for blocking the activity of TH1L antibody (MBS3213571)

Molecular Weight

66kDa

OMIM

605297

3D Structure

ModBase 3D Structure for Q8IXH7

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human TH1L

Product Categories/Family for anti-NELFCD antibody

Polyclonal; Transcription Regulation; Cell Biology; DNA/RNA/Protein Interactions; Transcription Factors

Applications Tested/Suitable for anti-NELFCD antibody

Western Blot (WB)

Other Notes

Small volumes of anti-NELFCD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NELFCD purchase

MBS3213571 is an antibody targeting NELFCD. Also known as TH1L antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NELFCD. It may not necessarily be applicable to this product.

NCBI GI #

38372376

NCBI GeneID

51497

NCBI Accession #

NP_945327

UniProt Primary Accession #

Q8IXH7

UniProt Related Accession #

Q8IXH7

GenBank Nucleotide #

NM_198976

NCBI Official Full Name

Negative elongation factor C/D

NCBI Official Synonym Full Names

negative elongation factor complex member C/D

NCBI Official Symbol

NELFCD

NCBI Official Synonym Symbols

TH1; TH1L; NELF-C; NELF-D; HSPC130

UniProt Protein Name

Negative elongation factor C/D

UniProt Synonym Protein Names

TH1-like protein

Protein Family

Negative elongation factor

UniProt Gene Name

NELFCD

UniProt Synonym Gene Names

NELFD; TH1; TH1L; NELF-C/D

UniProt Entry Name

NELFD_HUMAN

NCBI Protein Information

negative elongation factor C/D

NCBI Summary for NELFCD

The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini. [provided by RefSeq, Jul 2008]

UniProt Comments for NELFCD

NELFCD: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is composed of 4 subunits: WHSC2/NELF-A, COBRA1/NELF-B, TH1L (isoform NELF-C or isoform NELF-D) and RDBP/NELF-E. Interacts with ARAF1. Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal and cardiac muscle, adrenal, thyroid, kidney and pancreas. Belongs to the NELF-D family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: nucleoplasm; membrane

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent

Research Articles on NELFCD

1. A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NELFCD antibody

Products by Pathway	Pathway Diagram
Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies	Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies	Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies	Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
Formation Of RNA Pol II Elongation Complex Pathway antibodies	Formation Of RNA Pol II Elongation Complex Pathway Diagram
Formation Of The Early Elongation Complex Pathway antibodies	Formation Of The Early Elongation Complex Pathway Diagram
Formation Of The HIV-1 Early Elongation Complex Pathway antibodies	Formation Of The HIV-1 Early Elongation Complex Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
HIV Infection Pathway antibodies	HIV Infection Pathway Diagram
HIV Life Cycle Pathway antibodies	HIV Life Cycle Pathway Diagram