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ATG4B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3213604

This is a rabbit polyclonal antibody against ATG4B. It was validated on Western Blot using a cell lysate as a positive control.Target Description: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.… More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATG4B, Polyclonal Antibody
Full Product Name
ATG4B antibody - N-terminal region
Product Gene Name
anti-ATG4B antibody
Product Synonym Gene Name
APG4B; AUTL1; MGC1353
Immunogen Sequence
Synthetic peptide located within the following region: WGCMLRCGQM IFAQALVCRH LGRDWRWTQR KRQPDSYFSV LNAFIDRKDS
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
ATG4B peptide (MBS3238545) is used for blocking the activity of ATG4B antibody (MBS3213604)
Molecular Weight
44kDa
OMIM
611338
3D Structure
ModBase 3D Structure for Q9Y4P1
Homology
Cow: 92%; Dog: 93%; Guinea Pig: 100%; Human: 100%; Mouse: 93%; Rabbit: 85%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human ATG4B

Product Categories/Family for anti-ATG4B antibody

Polyclonal; Transcription Factor; Various; Disease Related; Autophagy
Applications Tested/Suitable for anti-ATG4B antibody
Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry (IHC) of anti-ATG4B antibody

Rabbit Anti-ATG4B Antibody
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Protocol located in Reviews and Data.
Rabbit Anti-ATG4B Polyclonal Antibody – Cat# MBS3213604

Western Blot (WB) of anti-ATG4B antibody

WB Suggested Anti-ATG4B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Placenta
Rabbit Anti-ATG4B Polyclonal Antibody, Western Blot (WB) – Cat# MBS3213604

Western Blot (WB) of anti-ATG4B antibody

WB Suggested Anti-ATG4B antibody Titration: 1 ug/mL
Sample Type: Human heart
Rabbit Anti-ATG4B Polyclonal Antibody, Western Blot (WB) – Cat# MBS3213604

Other Notes

Small volumes of anti-ATG4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG4B purchase

MBS3213604 is an antibody targeting ATG4B. Also known as ATG4B antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG4B. It may not necessarily be applicable to this product.

NCBI GI #
47132611
NCBI GeneID
23192
NCBI Accession #
NP_037457
UniProt Primary Accession #
Q9Y4P1
UniProt Related Accession #
Q9Y4P1
GenBank Nucleotide #
NM_013325
NCBI Official Full Name
cysteine protease ATG4B isoform a
NCBI Official Synonym Full Names
autophagy related 4B cysteine peptidase
NCBI Official Symbol
ATG4B
NCBI Official Synonym Symbols
APG4B; AUTL1
UniProt Protein Name
Cysteine protease ATG4B
UniProt Synonym Protein Names
AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B
Protein Family
Cysteine protease
UniProt Gene Name
ATG4B
UniProt Synonym Gene Names
APG4B; AUTL1; KIAA0943; hAPG4B
UniProt Entry Name
ATG4B_HUMAN
NCBI Protein Information
cysteine protease ATG4B

NCBI Summary for ATG4B

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for ATG4B

ATG4B: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Protease; EC 3.4.22.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: protein delipidation; mitochondrion degradation; positive regulation of protein catabolic process; autophagy; C-terminal protein lipidation; protein processing; protein targeting to membrane; proteolysis; positive regulation of autophagy; autophagic vacuole formation; cellular response to nitrogen starvation
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Research Articles on ATG4B

1. ATG4B expression was observed markedly upregulated by EWS-FLI1 overexpression, and silencing of ATG4B dramatically inhibits autophagy in Ewing sarcoma cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATG4B antibody

 Reference Product  PubMed Publications
 GABARAP antibody  >8 publications with ATG4B and GABARAP
 GABARAPL2 antibody  >6 publications with ATG4B and GABARAPL2
 MAP1LC3B antibody  >2 publications with ATG4B and MAP1LC3B

Pathways associated with anti-ATG4B antibody

 Products by Pathway  Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram

Diseases associated with anti-ATG4B antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with ATG4B and Neoplasms
 Inflammation Antibodies  >3 publications with ATG4B and Inflammation
 Bone Diseases Antibodies  >2 publications with ATG4B and Bone Diseases
 Nervous System Diseases Antibodies  >1 publications with ATG4B and Nervous System Diseases
 Drug Toxicity Antibodies  >1 publications with ATG4B and Drug Toxicity
 Carcinoma, Hepatocellular Antibodies  >1 publications with ATG4B and Carcinoma, Hepatocellular
 Necrosis Antibodies  >1 publications with ATG4B and Necrosis

Organs/Tissues associated with anti-ATG4B antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >8 publications with ATG4B and Muscle
 Bone Antibodies  >3 publications with ATG4B and Bone
 Nerve Antibodies  >2 publications with ATG4B and Nerve
 Eye Antibodies  >2 publications with ATG4B and Eye
 Liver Antibodies  >2 publications with ATG4B and Liver
 Lung Antibodies  >2 publications with ATG4B and Lung
 Bone Marrow Antibodies  >2 publications with ATG4B and Bone Marrow
 Brain Antibodies  >2 publications with ATG4B and Brain
 Heart Antibodies  >1 publications with ATG4B and Heart
 Kidney Antibodies  >1 publications with ATG4B and Kidney
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