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HSPA1A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3215047

This is a rabbit polyclonal antibody against Hspa1a. It was validated on Western BlotTarget Description: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides… More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hspa1a, Polyclonal Antibody
Full Product Name
Hspa1a antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
Hsp70-3; Hsp70.3; Hsp72; MGC189852; hsp68; hsp70A1
Immunogen Sequence
Synthetic peptide located within the following region: KMKEIAEAYL GHPVTNAVIT VPAYFNDSQR QATKDAGVIA GLNVLRIINE
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Dog, Goat, Human, Mouse, Rat, Yeast, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
Hspa1a peptide (MBS3239964) is used for blocking the activity of Hspa1a antibody (MBS3215047)
Molecular Weight
70kDa
3D Structure
ModBase 3D Structure for Q61696
Homology
Dog: 100%; Goat: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%
Applications Tested/Suitable for anti-HSPA1A antibody
Western Blot (WB)

Western Blot (WB) of anti-HSPA1A antibody

Lanes:
Lane1: 10ug mouse cortex brain lysate
Lane2: 25ug mouse cortex brain lysate
Lane3: 40ug mouse cortex brain lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:2000
Gene Name:
Hspa1a
Submitted by:
Anonymous
Rabbit Anti-HSPA1A Polyclonal Antibody – Cat# MBS3215047

Western Blot (WB) of anti-HSPA1A antibody

WB Suggested Anti-Hspa1a Antibody
Positive Control: Lane 1: 80ug pig serum
Primary Antibody Dilution : 1:1000
Secondary Antibody : Anti-rabbit-HRP
Secondry Antibody Dilution : 1:1000
Submitted by: Martina Ondrovics, University of Veterinary Medicine Vienna
Rabbit Anti-HSPA1A Polyclonal Antibody, Western Blot (WB) – Cat# MBS3215047

Western Blot (WB) of anti-HSPA1A antibody

WB Suggested Anti-Hspa1a Antibody
Positive Control: Lane 1: 80ug pig serum
Primary Antibody Dilution : 1:1000
Secondary Antibody : Anti-rabbit-HRP
Secondry Antibody Dilution : 1:1000
Submitted by: Martina Ondrovics, University of Veterinary Medicine Vienna
Rabbit Anti-HSPA1A Polyclonal Antibody, Western Blot (WB) – Cat# MBS3215047

Western Blot (WB) of anti-HSPA1A antibody

WB Suggested Anti-Hspa1a Antibody
Titration: 1.0 ug/ml
Positive Control: Mouse Heart
Rabbit Anti-HSPA1A Polyclonal Antibody, Western Blot (WB) – Cat# MBS3215047

Other Notes

Small volumes of anti-HSPA1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSPA1A purchase

MBS3215047 is an antibody targeting HSPA1A. Also known as Hspa1a antibody - N-terminal region. Reactive in Dog, Goat, Human, Mouse, Rat, Yeast, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HSPA1A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
heat shock 70 kDa protein 1A
NCBI Official Synonym Full Names
heat shock protein 1A
NCBI Official Symbol
Hspa1a
NCBI Official Synonym Symbols
Hsp72; hsp68; Hsp70-3; Hsp70.3; hsp70A1
UniProt Protein Name
Heat shock 70 kDa protein 1A
UniProt Synonym Protein Names
Heat shock 70 kDa protein 3; HSP70.3; Hsp68
UniProt Gene Name
Hspa1a
UniProt Synonym Gene Names
Hsp70-3; Hsp70A1; HSP70.3
NCBI Protein Information
heat shock 70 kDa protein 1A

UniProt Comments for HSPA1A

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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