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PDE5A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3215939

MBS3215939 is an antibody targeting PDE5A. Also known as PDE5A antibody - C-terminal region. Reactive in Tested: Human; Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit. More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDE5A, Polyclonal Antibody
Full Product Name
PDE5A antibody - C-terminal region
Product Gene Name
anti-PDE5A antibody
Product Synonym Gene Name
CGB-PDE; CN5A; PDE5; PDE5A1
Product Synonym Names
CGB-PDE, CN5A, PDE5, PDE5A1
Immunogen Sequence
Synthetic peptide located within the following region: TACDLSAITK PWPIQQRIAE LVATEFFDQG DRERKELNIE PTDLMNREKK
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Tested: Human; Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Concentration
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml (lot specific)
Preparation and Storage
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
PDE5A peptide (MBS3240841) is used for blocking the activity of PDE5A antibody (MBS3215939)
Molecular Weight
93kDa
OMIM
AF043731 mRNA
3D Structure
ModBase 3D Structure for O76074
Protein Size (# AA)
823 amino acids
Protein Interactions
UBC; PRKG1; CASP3;
Blocking Peptide
For anti-PDE5A (MBS596012) antibody is Catalog # MBS3240841
Predicted Homology Based on Immunogen Sequence
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%; Zebrafish: 82%
Replacement Item
This antibody may replace item sc-129427 from Santa Cruz Biotechnology.

Product Categories/Family for anti-PDE5A antibody

Polyclonal; Drugs and Drug Metabolism; Various; Hypoxia
Applications Tested/Suitable for anti-PDE5A antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-PDE5A antibody

Sample Type: Human placental tissue
Primary Antibody Dilution: 1:100
Secondary Antibody: Goat anti rabbit-HRP
Secondary Antibody Dilution: 1:10,000
Color/Signal Descriptions:
Brown: PDE5A
Purple: Haemotoxylin
Gene Name: PDE5A
Submitted by: Dr. Hiten D. Mistry and Anna Czajka, King's College London; Lesia Kurlak, University of Nottingham
Rabbit Anti-PDE5A Polyclonal Antibody – Cat# MBS3215939

Western Blot (WB) of anti-PDE5A antibody

WB Suggested Anti-PDE5A Antibody
Titration: 1.0 ug/ml
Positive Control: Placenta
Rabbit Anti-PDE5A Polyclonal Antibody, Western Blot (WB) – Cat# MBS3215939

Other Notes

Small volumes of anti-PDE5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE5A. It may not necessarily be applicable to this product.

NCBI GI #
61744432
NCBI GeneID
8654
NCBI Accession #
NP_246273
UniProt Primary Accession #
O76074
UniProt Related Accession #
O76074
GenBank Nucleotide #
NM_033437
NCBI Official Full Name
cGMP-specific 3',5'-cyclic phosphodiesterase isoform 3
NCBI Official Synonym Full Names
phosphodiesterase 5A
NCBI Official Symbol
PDE5A
NCBI Official Synonym Symbols
CN5A; PDE5; CGB-PDE
UniProt Protein Name
cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Synonym Protein Names
cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE
UniProt Gene Name
PDE5A
UniProt Synonym Gene Names
PDE5; CGB-PDE
NCBI Protein Information
cGMP-specific 3',5'-cyclic phosphodiesterase

NCBI Summary for PDE5A

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for PDE5A

PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.

Protein type: EC 3.1.4.35; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; cGMP binding

Biological Process: cGMP catabolic process
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Research Articles on PDE5A

1. Thsese findings provide insight into the existence of distinct "pools" of PDE5A in human arterial smooth muscle cells and support the idea that these discrete compartments regulate distinct cGMP-dependent events.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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