Rabbit Anti-PDHA2 Polyclonal Antibody – Cat# MBS3222835

Host: Rabbit
Target Name: PDHA2
Sample Tissue: Human LN18 Whole Cell lysates
Antibody Dilution: 1ug/ml

PDHA2 Antibody – Rabbit Polyclonal

Name: PDHA2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3222835
Price: 490 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3222835

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle. More details

$490 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PDHA2, Polyclonal Antibody

Full Product Name

PDHA2 Antibody - middle region

Product Gene Name

anti-PDHA2 antibody

Product Synonym Gene Name

PDHAL

Immunogen Sequence

Synthetic peptide located within the following region: MELQTYRYHG HSMSDPGVSY RTREEIQEVR SKRDPIIILQ DRMVNSKLAT

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Purity/Purification

Affinity purified

Preparation and Storage

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Antibody/Peptide Pairs

PDHA2 peptide (MBS3247502) is used for blocking the activity of PDHA2 antibody (MBS3222835)

Molecular Weight

42 kDa

OMIM

300502

3D Structure

ModBase 3D Structure for P08559

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human PDHA2

Product Categories/Family for anti-PDHA2 antibody

Polyclonal; Cell Biology

Applications Tested/Suitable for anti-PDHA2 antibody

Western Blot (WB)

Other Notes

Small volumes of anti-PDHA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDHA2 purchase

MBS3222835 is an antibody targeting PDHA2. Also known as PDHA2 Antibody - middle region. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PDHA2. It may not necessarily be applicable to this product.

NCBI GI #

4885543

NCBI GeneID

5161

NCBI Accession #

NP_005381.1

UniProt Primary Accession #

P08559

UniProt Related Accession #

P29803

GenBank Nucleotide #

NM_005390.4

NCBI Official Full Name

pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial

NCBI Official Synonym Full Names

pyruvate dehydrogenase E1 alpha 2 subunit

NCBI Official Symbol

PDHA2

NCBI Official Synonym Symbols

PDHAL

UniProt Protein Name

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

UniProt Synonym Protein Names

PDHE1-A type I

Protein Family

Pyruvate dehydrogenase E1 component

UniProt Gene Name

PDHA1

UniProt Synonym Gene Names

PHE1A

UniProt Entry Name

ODPA_HUMAN

NCBI Protein Information

pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial

UniProt Comments for PDHA2

PDHA1: a mitochondrial matrix enzyme that catalyzes the oxidative decarboxylation of pyruvate, producing acetyl-CoA and CO2. A key enzyme in controlling the balance between lipid and glucose oxidation depending on substrate availability. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. The activity of PDH is tightly regulated by phosphorylation. The phosphorylation of at least one of three specific serine residues in E1 subunit by PDHK inactivates the PDHC, while dephosphorylation by PDP restores its activity. Sites 1, 2, and 3 of PDHA1 are S293, S300, and S232, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - butanoate; Oxidoreductase; EC 1.2.4.1; Carbohydrate Metabolism - pyruvate; Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis

Chromosomal Location of Human Ortholog: Xp22.1

Cellular Component: mitochondrion; mitochondrial matrix; pyruvate dehydrogenase complex; nucleus

Molecular Function: pyruvate dehydrogenase activity; pyruvate dehydrogenase (acetyl-transferring) activity

Biological Process: cellular metabolic process; acetyl-CoA biosynthetic process from pyruvate; glycolysis; tricarboxylic acid cycle; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; pyruvate metabolic process

Disease: Pyruvate Dehydrogenase E1-alpha Deficiency

Research Articles on PDHA2

1. Our findings show that also recessive complex digit malformation can be caused by BMPR1B variant and not all biallelic BMPR1B variants cause acromesomelic dysplasia. PDHA2 is a novel candidate gene for male infertility; the protein product is a mitochondrial enzyme with highest expression in ejaculated sperm.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDHA2 antibody

Reference Product	PubMed Publications
LDHC antibody	>1 publications with PDHA2 and LDHC
DLD antibody	>1 publications with PDHA2 and DLD
PDHB antibody	>1 publications with PDHA2 and PDHB

Pathways associated with anti-PDHA2 antibody

Products by Pathway	Pathway Diagram
Acetylcholine Synthesis Pathway antibodies	Acetylcholine Synthesis Pathway Diagram
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Central Carbon Metabolism In Cancer Pathway antibodies	Central Carbon Metabolism In Cancer Pathway Diagram
Central Carbon Metabolism In Cancer Pathway antibodies	Central Carbon Metabolism In Cancer Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Glucagon Signaling Pathway antibodies	Glucagon Signaling Pathway Diagram
Glucagon Signaling Pathway antibodies	Glucagon Signaling Pathway Diagram
Glycolysis / Gluconeogenesis Pathway antibodies	Glycolysis / Gluconeogenesis Pathway Diagram

Diseases associated with anti-PDHA2 antibody

Disease Name	Pubmed Publications
Chromosome Aberrations Antibodies	>1 publications with PDHA2 and Chromosome Aberrations

Organs/Tissues associated with anti-PDHA2 antibody

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>7 publications with PDHA2 and Testis