TCF4 Antibody – Rabbit Polyclonal

Function: Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Isoform 2 inhibits MYOD1 activation of the cardiac alpha-actin promoter. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. May have a regulatory function in developmental processes as well as during neuronal plasticity. Ref.7

Subunit structure: Efficient DNA binding requires dimerization with another bHLH protein. Isoform 2 seems to form inactive heterodimers with MYOD1. The CTNNB1 and TCF4 complex interacts with PML. Identified in a complex with CTNNB1 and FERMT2

By similarity. Interacts with HIVEP2. Interacts with NEUROD2. Ref.6 Ref.7

Subcellular location: Nucleus

Probable.

Tissue specificity: Expressed in the cerebral cortex, Purkinje and granule cell layers of the cerebellum, olfactory neuroepithelium, pyramidal cells of hippocampal layers CA1-CA4, and in the granular cells of the dentate gyrus.

Developmental stage: Expressed in proliferative zones during development and in the adult in areas of neuronal plasticity. At embryonic day 12 (E12), expression is localized in the cortex, cerebellum, pons, medulla and spinal cord. From E18 to adulthood, high levels of expression are found in the pyramidal cells of hippocampal layers CA1-CA4, and in the granular cells of the dentate gyrus. At postnatal day 7, expression is high in the visual cortex and in the subependymal region extending from the anterior lateral ventricle into the olfactory bulb.

Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.