Collagen Type X (COL10) ELISA Kit for Human

Name: Human COL10 ELISA Kit; Collagen Type X; MyBioSource
Brand: MyBioSource
SKU: MBS032637
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS032637

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of COL10 (hereafter termed "analyte") in undiluted… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Collagen Type X (COL10), ELISA Kit

Full Product Name

Human Collagen Type X ELISA Kit

Product Gene Name

COL10 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Detection Range

3.12ng/ml-100ng/ml

Sensitivity

1.0ng/ml

Specificity

No significant cross-reactivity or interference between Human COL10 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

66,158 Da

OMIM

120110

3D Structure

ModBase 3D Structure for Q03692

Other Notes

Small volumes of COL10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COL10 purchase

MBS032637 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Type X (COL10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COL10. The ELISA analytical biochemical technique of the MBS032637 kit is based on COL10 antibody-COL10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for COL10. It may not necessarily be applicable to this product.

NCBI GI #

119568625

NCBI GeneID

1300

NCBI Accession #

EAW48240.1

UniProt Primary Accession #

Q03692

UniProt Secondary Accession #

A1L4P2

UniProt Related Accession #

Q03692

NCBI Official Full Name

collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia)

NCBI Official Synonym Full Names

collagen, type X, alpha 1

NCBI Official Symbol

COL10A1

UniProt Protein Name

Collagen alpha-1(X) chain

Protein Family

Zinc finger protein CONSTANS

UniProt Gene Name

COL10A1

UniProt Entry Name

COAA1_HUMAN

NCBI Protein Information

collagen alpha-1(X) chain; collagen alpha-1(X) chain; collagen X, alpha-1 polypeptide; Schmid metaphyseal chondrodysplasia

NCBI Summary for COL10

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]

UniProt Comments for COL10

COL10A1: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage. Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD). SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q21-q22

Cellular Component: proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; extracellular region; cell cortex

Molecular Function: metal ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; cartilage development; skeletal development; endochondral ossification

Disease: Metaphyseal Chondrodysplasia, Schmid Type

Research Articles on COL10

1. The results show that COL10A1 is a tumor biomarker upregulated in a wide variety of tumors including those of the breast, colon, bladder, stomach, esophagus, lung, testis, ovary and pancreas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with COL10 elisa kit

Products by Pathway	Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Collagen Biosynthesis And Modifying Enzymes Pathway antibodies	Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
Endochondral Ossification Pathway antibodies	Endochondral Ossification Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Senescence And Autophagy Pathway antibodies	Senescence And Autophagy Pathway Diagram

Diseases associated with COL10 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>11 publications with COL10 and Neoplasms
Congenital Abnormalities Antibodies	>7 publications with COL10 and Congenital Abnormalities
Necrosis Antibodies	>5 publications with COL10 and Necrosis
Craniofacial Abnormalities Antibodies	>4 publications with COL10 and Craniofacial Abnormalities
Inflammation Antibodies	>3 publications with COL10 and Inflammation
Abnormalities, Multiple Antibodies	>3 publications with COL10 and Abnormalities, Multiple
Embryo Loss Antibodies	>3 publications with COL10 and Embryo Loss
Metaphyseal chondrodysplasia Schmid type Antibodies	>3 publications with COL10 and Metaphyseal chondrodysplasia Schmid type
Kidney Diseases Antibodies	>2 publications with COL10 and Kidney Diseases
Fibrosis Antibodies	>2 publications with COL10 and Fibrosis

Organs/Tissues associated with COL10 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>146 publications with COL10 and Bone
Connective Tissue Antibodies	>61 publications with COL10 and Connective Tissue
Blood Antibodies	>8 publications with COL10 and Blood
Eye Antibodies	>3 publications with COL10 and Eye
Lung Antibodies	>2 publications with COL10 and Lung
Uncharacterized Tissue Antibodies	>1 publications with COL10 and Uncharacterized Tissue