Immunoglobulin G2 (IgG2) ELISA Kit for Bovine

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of IgG2 (hereafter termed "analyte") in undiluted original Bovine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

Small volumes of IgG2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS032764 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Immunoglobulin G2 (IgG2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IgG2. The ELISA analytical biochemical technique of the MBS032764 kit is based on IgG2 antibody-IgG2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IgG2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IgG2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). For detection of bovine serum plasma biological samples or cell tissue homogenates, an enzyme linked immunosorbent assay ( ELISA ) is available utilizing guinea pig and mouse antibodies for high level precision, ng ml sensitivity and improved intra assay and inter assay variability. ELISA kit includes recombinant immunoglobulin G2 protein for standard curve development, product ELISA plate, sample reagents in a ready set go format, ideal for quantitative research into bovine samples middle high level immunoglobulin G2 protein expression. The primary antibody in the ELISA kits shows no cross-reactivity of IgG2 tested in homo sapiens human IgG fractions, mouse IgG2a or other bovine IgG subtypes. Utilizing an antibody immunoglobulin enzymatic colorimetric or fluorescent ALEXA 174 approach to detect protein levels, assay detection ranges of 0.25 mg/ml - 8 mg/ml and sensitivity down to 0.1mg/ml can be achieved reproducibly. ELISA ready products are shipped with MSDS and ELISA pubmed searches included.