Methylated Lysine Antibody – Rabbit Polyclonal

Background Info: Detects proteins containing methylated lysine residues in SDS PAGE immunoblots.

Scientific Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).

1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Melcher M. et al. (2000). Mol Cell Biol 20: 3728- 3741. 3. Bannister AJ. et al. (2001). Nature 410(6824): 120-4. 4. Im H. et al. (2003). J Biol Chem. 278: 18346-18352. 5. Ng H.H., et al. (2002). J Biol Chem. 277: 34655-34657. 6. Zegerman P., et al. (2002). J Biol Chem. 277: 11621- 11624.

Small volumes of anti-Methylated Lysine antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS805807 is an antibody targeting Methylated Lysine. Also known as Methylated Lysine Antibody: HRP. Reactive in Multi species. Host: Rabbit.