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HIS TAG Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS330124

Poly histidine (HHHHHH) is frequently used as affinity tag to facilitate the detection, isolation, and localaiztion of the recombinant proteins. This high affinity and specific anti-Histidine antibody is developed using HHHHHH peptide conjugated to KLH and… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
His Tag, Polyclonal Antibody
Full Product Name
Anti-His Tag pAb (Rabbit)
Product Gene Name
Product Synonym Names
Anti-His Tag Antibody(Rabbit Polyclonal.)
Clonality
Polyclonal
Host
Rabbit
Form/Format
1 mg /mL in TBS with 0.01% NaN3.
Purity/Purification
Affinity purified with HHHHHHH peptide on Agarose
Specificity
Recognize His-tag proteins in all species.
Preparation and Storage
Store at -20 degree C, stable for up to three years.
Immunogen
Polyhistidine peptide HHHHHH conjugated to KLH
Quality Control
Western blot analysis of the His-tag protein in the lysate of bacterial express of a His-tagged protein.
Applications Tested/Suitable for anti-His Tag antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes for anti-His Tag antibody
ELISA: 0.5 ug/mL, WB:1 ug/mL, IP:5 ug/mg crude protein, IF:2 ug/mL.The investigator must determine the ideal working concentration for each specific application.

Testing Data of anti-His Tag antibody

Lane 1 and 4: MW marker
Rabbit Anti-HIS TAG Polyclonal Antibody – Cat# MBS330124

Other Notes

Small volumes of anti-His Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for His Tag purchase

MBS330124 is an antibody targeting His Tag. Also known as Anti-His Tag pAb (Rabbit). Host: Rabbit.
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