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CD99 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS335274

MBS335274 is an antibody targeting CD99. Also known as Mouse Anti-Human CD99 Monoclonal Antibody, PE Conjugated Clone B-N24. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD99, Monoclonal Antibody
Full Product Name
Mouse Anti-Human CD99 Monoclonal Antibody, PE Conjugated Clone B-N24
Product Gene Name
Product Synonym Names
CD99
Clonality
Monoclonal
Isotype
IgG1
Clone Number
B-N24
Host
Mouse
Species Reactivity
Human
Form/Format
Phosphate-buffered saline with 5% BSA and 0.1% sodium azide
Purity/Purification
Ion exchange chromatography
Specificity
Recognises a 32 kDa protein
Preparation and Storage
Stable at +2-8 degree C for six months after reconstitution. DO NOT FREEZE.
Molecular Weight
17,128 Da
OMIM
313470
3D Structure
ModBase 3D Structure for P14209
Antigen
CD99
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
HSB2 T cell line
Conjugation
PE
Applications Tested/Suitable for anti-CD99 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD99 antibody
Working Dilution: Reconstitutes with 1 ml deionised water. Use 10mul to label 106 cells or 100 mul of whole blood

Other Notes

Small volumes of anti-CD99 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD99. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD99 antigen isoform a
NCBI Official Synonym Full Names
CD99 molecule
NCBI Official Symbol
CD99
NCBI Official Synonym Symbols
MIC2; HBA71; MIC2X; MIC2Y; MSK5X
UniProt Protein Name
CD99 antigen
UniProt Synonym Protein Names
12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD_antigen: CD99
Protein Family
UniProt Gene Name
CD99
UniProt Synonym Gene Names
MIC2; MIC2X; MIC2Y
UniProt Entry Name
CD99_HUMAN
NCBI Protein Information
CD99 antigen

NCBI Summary for CD99

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013]
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UniProt Comments for CD99

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane

Biological Process: cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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