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CD1b/c Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS335351

MBS335351 is an antibody targeting CD1b/c. Also known as Mouse Anti-Human CD1b/c Monoclonal Antibody, FITC Conjugated Clone B-B5. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD1b/c, Monoclonal Antibody
Full Product Name
Mouse Anti-Human CD1b/c Monoclonal Antibody, FITC Conjugated Clone B-B5
Product Gene Name
Product Synonym Names
CD1b/c
Clonality
Monoclonal
Isotype
IgG1
Clone Number
B-B5
Host
Mouse
Species Reactivity
Human
Form/Format
Phosphate-buffered saline with 5% BSA and 0.09% sodium azide
Purity/Purification
Ion exchange chromatography
Specificity
Recognises a 43-45 kDa protein
Preparation and Storage
Store at +2-8 degree C for 12 months. For longer storage, freeze aliquots.
Matching Pairs
Unconjugated Azide Free Antibody: CD1b/c Clone #B-B5 (MBS335070)
FITC Conjugated Antibody: CD1b/c Clone #B-B5 (MBS335351)
Matching Pairs
FITC Conjugated Antibody: CD1b/c Clone #B-B5 (MBS335351)
Unconjugated Antibody: CD1b/c Clone #B-B5 (MBS335151)
Molecular Weight
30,740 Da
OMIM
188360
3D Structure
ModBase 3D Structure for P29016
Antigen
CD1b/c
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
Thymus cells and Jurkat cell line
Conjugation
FITC
Applications Tested/Suitable for anti-CD1b/c antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD1b/c antibody
Working Dilution: Use 10 mul to label 106 cells or 100 mul of whole blood

Testing Data of anti-CD1b/c antibody

A typical staining pattern with the B-B5 monoclonal antibody of Molt 4 cell line
Mouse Anti-CD1b/c Monoclonal Antibody – Cat# MBS335351

Product References and Citations for anti-CD1b/c antibody

Bakri et.al J Immunol 2001

Other Notes

Small volumes of anti-CD1b/c antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD1b/c. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
CD1b antigen
NCBI Official Synonym Full Names
CD1b molecule
NCBI Official Symbol
CD1B
NCBI Official Synonym Symbols
R1; CD1; CD1A
UniProt Protein Name
T-cell surface glycoprotein CD1b
UniProt Synonym Protein Names
CD_antigen: CD1b
UniProt Gene Name
CD1B
UniProt Entry Name
CD1B_HUMAN
NCBI Protein Information
T-cell surface glycoprotein CD1b

NCBI Summary for CD1b/c

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD1b/c

CD1B: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cell surface; lysosomal membrane; integral to membrane; plasma membrane; endosome membrane

Molecular Function: protein binding; beta-2-microglobulin binding; exogenous lipid antigen binding; endogenous lipid antigen binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; immune response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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