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COVID 19 Spike Glycoprotein S1 RBD Coronavirus (COVID-19) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS335828

Spike protein (S protein) is one of four structural proteins of Coronavirus (SARS-Cov, SARS-Cov-2, MERS amongst other), S protein plays the most important role in viral attachment, fusion and entry, and it serves as a target for development of antibodies,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
COVID 19 Spike Glycoprotein S1 RBD Coronavirus (COVID-19), Active Protein
Full Product Name
Recombinant Human SARS-CoV-2 Spike Glycoprotein S1 RBD (CHO)
Product Gene Name
Product Synonym Gene Name
2019-nCoV; SARS-CoV-2; SARS coronavirus 2 proteome; COVID19; SARS-CoV2
Product Synonym Names
2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein S1 RBD; Receptor Binding Domain
Sequence
Arg319-Phe 541
Host
CHO Cells
Form/Format
Liquid ; PBS
Purity/Purification
Affinity chromatography. Purity: >95% by SDS PAGE
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze-thaw cycles Stable one week at 2 to 8 degree C after reconstitution under sterile conditions Stable 12 months -20 degree C to -80 degree C after reconstitution under sterile conditions
Molecular Weight
Predicted Molecular Weight: 26.1 kDa; SDS Page: Approx. 30 kDa
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Tag Information
HIS Tag C-Terminus
Extinction Coefficient
33850 M-1.cm-1 Abs 0.1% (=1 g/l) 1.29 assuming all pairs of Cys residues form cystines
Endotoxins
Negative on cell-based assay
Activity
Active form: positive binding with recombinant ACE2 Human. Recognized by CR3022 anti RBD recombinant antibody and Covid-19 positive patients serum samples.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

SDS-Page of COVID-19 active protein

SDS-PAGE 4-15% under reducing conditions and visualized by Coomassie blue staining showing a band at approx. 30 kDa
COVID-19 Active Protein – Cat# MBS335828

Western Blot (WB) of COVID-19 active protein

Western Blot: Spike RBD HIS Tag detected with Anti-HIS-Tag antibody HRP conjugate showing a band at approx. 30 kDa
COVID-19 Active Protein, Western Blot (WB) – Cat# MBS335828

Other Notes

Small volumes of COVID-19 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COVID-19 purchase

MBS335828 is an active protein targeting COVID-19. Also known as Recombinant Human SARS-CoV-2 Spike Glycoprotein S1 RBD (CHO). Host: CHO Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for COVID-19. It may not necessarily be applicable to this product.

NCBI Accession #

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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