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Interleukin-2 (IL-2) Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS335849

IL-2 is a powerful immunoregulatory lymphokine produced by T-Cells in response to antigenic or mitogenic activation (1). IL-2 stimulates growth and differentiation of B-Cells, most T-cells, NK cells, monocytes and macrophages (2,3,4). Mature IL-2 is a 15.4kDa… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Interleukin-2 (IL-2), Active Protein
Full Product Name
Recombinant Human Interleukin-2 (IL-2)
Product Gene Name
Host
HEK 293 cells
Form/Format
Lyophilized from PBS pH 7.4 (NaCl 155mM, Na2HPO4 8mM & KH2PO4, 1.8mM, Trehalose) with BSA as carrier protein
Purity/Purification
Purity: >95% by SDS PAGE gel
Purification: Affinity chromatography followed by Ion exchange chromatography
Concentration
10?g / vial – 125 ?g/ml (715 H02.010BSA) 25 ?g/ vial – 125 ?g/ml (715 H02.025BSA) (lot specific)
Preparation and Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Stable one week at 2 to 8°C after reconstitution under sterile conditions.
Stable 12 months -20°C to -80°C after reconstitution under sterile conditions.
Molecular Weight
Predicted Molecular Weight: 16354.96 Da
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Souce Note
Human IL-2 produced in HEK 293 cells
Tag
His Tag C-Terminus
Extinction Coefficient
10 095 M -1 .cm -1, Abs 0.1%(=1 g/l) 0.617 assuming all pairs residues from cysteines
Reconstitution
Centrifuge the vial prior opening It is recommended to reconstitute in sterile water at 100 ug/ml
Endotoxins
Negative on cell based assay
Activity
Measured in a cell based assay on stimulated Kit225 cells (EC50= 0.2 ng/ml)
Application Notes for IL-2 active protein
SDS-PAGE: SDS Page 17 KDa & 18 KDa, reducing conditions ; second band results of glycosylation (confirmed by mass spectrometry)

SDS-PAGE (SDS) of IL-2 active protein

SDS-PAGE under reducing conditions and visualized by Coomassie blue staining showing a band at 17 KDa and one at 18 KDa corresponding to the glycosylated form. Western Blot: IL-2 as antigen (with mouse monoclonal Antibody B-G5 # MBS335411) chemiluminescence detection
Human IL-2 Active Protein – Cat# MBS335849

Testing Data of IL-2 active protein

Biological Activity measured in a cell proliferation assay using Kit225 cells.The EC50 for this effect is 0.2 ng/ml
Human IL-2 Active Protein, Testing Data – Cat# MBS335849

Other Notes

Small volumes of IL-2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-2 purchase

MBS335849 is an active protein targeting IL-2. Also known as Recombinant Human Interleukin-2 (IL-2). Host: HEK 293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-2. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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