Mouse Anti-PD-1 Monoclonal Antibody – Cat# MBS344001

PD-1 [IHC001] on Tonsil

PD-1 Antibody – Mouse Monoclonal

Name: PD-1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS344001
Price: 215 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS344001

Intended Use: The PD-1 [IHC001] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test… More details

$215 / 0.1 mL (Concentrate) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PD-1, Monoclonal Antibody

Full Product Name

PD-1

Product Gene Name

anti-PD-1 antibody

Clonality

Monoclonal

Host

Mouse

Species Reactivity

Human

Form/Format

Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide

Preparation and Storage

Store at 2-8 degree C. Do not freeze.
To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.
Positive and negative controls should be simultaneously run with unknown specimens.

Molecular Weight

31,647 Da

3D Structure

ModBase 3D Structure for Q15116

Positive Control

Tonsil, Lymph Node

Principles and Procedures

Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.

Reconstitution

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.

Specimen Collection and Preparation for Analysis

Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).
Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.

Application Notes for anti-PD-1 antibody

The recommended working dilution range is 1:100 - 1:200.

Product References and Citations for anti-PD-1 antibody

1. Dorfman DM, et al. Am J Surg Pathol. 2006; 30:802-10.
2. Hamanishi J, et al. Proc Natl Acad Sci USA. 2007; 104:3360-5.
3. Kobayashi M, et al. J Rheumatol. 2005; 32:2156-63.
4. Konishi J, et al. Clin Cancer Res. 2004; 10:5094-100.
5. Mataki N, et al. Am J Gastroenterol. 2007; 102:302-12.
6. Kim JW, et al. Oncology (Williston Park). 2014; 28:15-28.
7. Tumeh PC, et al. Nature. 2014; 515:568-71.
8. D'Incecco A, et al. Br J Cancer. 2015; 112:95-102.
9. Tykodi SS. Onco Targets Ther. 2014; 7:1349-59.

Other Notes

Small volumes of anti-PD-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PD-1 purchase

MBS344001 is an antibody targeting PD-1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PD-1. It may not necessarily be applicable to this product.

NCBI GI #

167857792

NCBI GeneID

5133

NCBI Accession #

NP_005009.2

UniProt Primary Accession #

Q15116

UniProt Secondary Accession #

O00517; Q8IX89

UniProt Related Accession #

Q15116

GenBank Nucleotide #

NM_005018.2

NCBI Official Full Name

programmed cell death protein 1

NCBI Official Synonym Full Names

programmed cell death 1

NCBI Official Symbol

PDCD1

NCBI Official Synonym Symbols

PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1

UniProt Protein Name

Programmed cell death protein 1

UniProt Synonym Protein Names

CD_antigen: CD279

UniProt Gene Name

PDCD1

UniProt Synonym Gene Names

PD1; Protein PD-1; hPD-1

NCBI Protein Information

programmed cell death protein 1

NCBI Summary for PD-1

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

UniProt Comments for PD-1

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.

Research Articles on PD-1

1. Results indicated that high-level PD1 expression may be an important factor associated with the immune checkpoint pathway in liver cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PD-1 antibody

Reference Product	PubMed Publications
CD4 antibody	>418 publications with PD-1 and CD4
CD274 antibody	>336 publications with PD-1 and CD274
PDCD1LG2 antibody	>99 publications with PD-1 and PDCD1LG2
PTPN6 antibody	>7 publications with PD-1 and PTPN6
PTPN11 antibody	>7 publications with PD-1 and PTPN11
HLA-DR4 antibody	>1 publications with PD-1 and HLA-DR4

Pathways associated with anti-PD-1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Costimulation By The CD28 Family Pathway antibodies	Costimulation By The CD28 Family Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
PD-1 Signaling Pathway antibodies	PD-1 Signaling Pathway Diagram
T Cell Receptor Signaling Pathway antibodies	T Cell Receptor Signaling Pathway Diagram
T Cell Receptor Signaling Pathway antibodies	T Cell Receptor Signaling Pathway Diagram

Diseases associated with anti-PD-1 antibody

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>147 publications with PD-1 and Liver Diseases
Inflammation Antibodies	>115 publications with PD-1 and Inflammation
Melanoma Antibodies	>79 publications with PD-1 and Melanoma
Necrosis Antibodies	>75 publications with PD-1 and Necrosis
Lupus Erythematosus, Systemic Antibodies	>72 publications with PD-1 and Lupus Erythematosus, Systemic
Lung Diseases Antibodies	>58 publications with PD-1 and Lung Diseases
Lung Neoplasms Antibodies	>38 publications with PD-1 and Lung Neoplasms
Adenocarcinoma Antibodies	>35 publications with PD-1 and Adenocarcinoma
Kidney Diseases Antibodies	>28 publications with PD-1 and Kidney Diseases
Liver Neoplasms Antibodies	>26 publications with PD-1 and Liver Neoplasms

Organs/Tissues associated with anti-PD-1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>945 publications with PD-1 and Blood
Kidney Antibodies	>36 publications with PD-1 and Kidney