Mouse Anti-PAX-8 Monoclonal Antibody – Cat# MBS344007

PAX-8 [IHC008] on Thyroid Gland

PAX-8 Antibody – Mouse Monoclonal

Name: PAX-8 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS344007
Price: 285 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS344007

Intended Use: The PAX8 [IHC008] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test… More details

$285 / 0.1 mL (Concentrate) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PAX-8, Monoclonal Antibody

Full Product Name

PAX-8

Product Gene Name

anti-PAX-8 antibody

Product Synonym Names

PAX8

Clonality

Monoclonal

Host

Mouse

Species Reactivity

Human

Form/Format

Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide

Preparation and Storage

Store at 2-8 degree C. Do not freeze.
To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.
Positive and negative controls should be simultaneously run with unknown specimens.

Molecular Weight

6,524 Da

3D Structure

ModBase 3D Structure for Q99573

Positive Control

Ovarian Carcinoma (Non-Mucinous Carcinoma), Thyroid Carcinoma, Renal Cell Carcinoma

Principles and Procedures

Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.

Reconstitution

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.

Specimen Collection and Preparation for Analysis

Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).
Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.

Application Notes for anti-PAX-8 antibody

The recommended working dilution range is 1:100 - 1:200.

Product References and Citations for anti-PAX-8 antibody

1. Tong GX, et al. Mod Pathol. 2009; 22:1218-27.
2. Nonaka D, et al. Mod Pathol. 2008; 21:192-200.
3. Tamara L, et al. Am J Surg Pathol. 2009; 33:1037-41.
4. Nikiforova MN, et al. Am J Surg Pathol. 2002; 26:1016-23.
5. Nonaka D, et al. Am J Surg Pathol. 2008; 32:1566-71.
6. Marques AR, et al. J Clin Endocrinol Metab. 2002; 87:3947-52.
7. Tina DP, et al. J Biol Chem. 2003; 278:3395-3402.
8. Bowen NJ, et al. Gynecol Oncol. 2007; 104:331-7.
9. Zhang P, et al. Pathol Int. 2006; 56:240-245.

Other Notes

Small volumes of anti-PAX-8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAX-8 purchase

MBS344007 is an antibody targeting PAX-8. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PAX-8. It may not necessarily be applicable to this product.

NCBI GI #

1850829

NCBI GeneID

7849

NCBI Accession #

CAA67903.1

UniProt Primary Accession #

Q99573

UniProt Related Accession #

Q06710

NCBI Official Full Name

Pax-8, partial

NCBI Official Synonym Full Names

paired box 8

NCBI Official Symbol

PAX8

UniProt Protein Name

Pax-8 protein

UniProt Synonym Protein Names

Pax-8 protein

Protein Family

Paired box protein

UniProt Gene Name

Pax-8

NCBI Protein Information

paired box protein Pax-8

NCBI Summary for PAX-8

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Research Articles on PAX-8

1. PAX8-PPARG fusions may not play major roles in the tumorigenesis of paediatric follicular thyroid carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PAX-8 antibody

Products by Pathway	Pathway Diagram
Id Signaling Pathway antibodies	Id Signaling Pathway Diagram
Pathways In Cancer antibodies	Pathways In Cancer Diagram
TSH Signaling Pathway antibodies	TSH Signaling Pathway Diagram
Thyroid Cancer Pathway antibodies	Thyroid Cancer Pathway Diagram
Thyroid Cancer Pathway antibodies	Thyroid Cancer Pathway Diagram
Thyroid Hormone Synthesis Pathway antibodies	Thyroid Hormone Synthesis Pathway Diagram
Thyroid Hormone Synthesis Pathway antibodies	Thyroid Hormone Synthesis Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram

Organs/Tissues associated with anti-PAX-8 antibody

Organ/Tissue Name	Pubmed Publications
Thyroid Antibodies	>547 publications with PAX-8 and Thyroid
Kidney Antibodies	>187 publications with PAX-8 and Kidney
Brain Antibodies	>61 publications with PAX-8 and Brain
Lung Antibodies	>52 publications with PAX-8 and Lung
Ovary Antibodies	>49 publications with PAX-8 and Ovary
Lymph Node Antibodies	>36 publications with PAX-8 and Lymph Node
Bladder Antibodies	>21 publications with PAX-8 and Bladder
Cervix Antibodies	>18 publications with PAX-8 and Cervix
Eye Antibodies	>18 publications with PAX-8 and Eye
Pancreas Antibodies	>14 publications with PAX-8 and Pancreas