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CD45 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS344016

Intended Use: The CD45 (LCA) [IHC045] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD45(LCA), Monoclonal Antibody
Full Product Name
CD45(LCA)
Product Gene Name
anti-CD45 antibody
Clonality
Monoclonal
Host
Mouse
Species Reactivity
Human
Form/Format
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.
Positive and negative controls should be simultaneously run with unknown specimens.
Positive Control
Tonsil, Lymph Node, Lymphoma
Principles and Procedures
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
Reconstitution
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
Specimen Collection and Preparation for Analysis
Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).
Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
Application Notes for anti-CD45 antibody
The recommended working dilution range is 1:100 - 1:200.

Immunohistochemistry (IHC) of anti-CD45 antibody

CD45 (LCA) [IHC045] on Tonsil
Mouse Anti-CD45 Monoclonal Antibody – Cat# MBS344016

Product References and Citations for anti-CD45 antibody

1. Kurtin PJ, et al. Hum Pathol. 1985; 16:353-65.
2. Michels S, et al. Arch Pathol Lab Med. 1987; 111:1035-39.
3. Muzaffar S, et al. JPMA J Pak Med Assoc. 1997; 47:106-9.

Other Notes

Small volumes of anti-CD45 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD45 purchase

MBS344016 is an antibody targeting CD45. Also known as CD45(LCA). Reactive in Human. Host: Mouse.
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