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MSH2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS344032

Intended Use: The MSH2 [IHC410] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MSH2, Monoclonal Antibody
Full Product Name
MSH2
Product Gene Name
anti-MSH2 antibody
Clonality
Monoclonal
Host
Mouse
Species Reactivity
Human
Form/Format
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.
Positive and negative controls should be simultaneously run with unknown specimens.
Molecular Weight
107,754 Da
Positive Control
Colon Mucosa, Colon Carcinoma
Principles and Procedures
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
Reconstitution
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
Specimen Collection and Preparation for Analysis
Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).
Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
Application Notes for anti-MSH2 antibody
The recommended working dilution range is 1:100 - 1:200.

Immunohistochemistry (IHC) of anti-MSH2 antibody

MSH2 [IHC410] on Esophagus
Mouse Anti-MSH2 Monoclonal Antibody – Cat# MBS344032

Product References and Citations for anti-MSH2 antibody

1. Pal T, et al. Cancer. 2008; 113:733-42.
2. Brueckl WM, et al. Anticancer Res. 2003; 23:1773-8.
3. Rigau V, et al. Arch Pathol Lab Med. 2003; 127:694-700.
4. Renkonen E, et al. J Clin Oncol. 2003; 21:3629-37.
5. Hoedema R, et al. Am Surg. 2003; 69:387-92.
6. Christensen M, et al. Cancer. 2002; 95:2422-30.
7. Wahlberg SS, et al. Cancer Res. 2002; 62:3485-92.
8. Lanza G, et al. Mod Pathol. 2002; 15:741-9.
9. Thibodeau SN, et al. Cancer Res 1996; 56:4836-40.

Other Notes

Small volumes of anti-MSH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSH2 purchase

MBS344032 is an antibody targeting MSH2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MSH2. It may not necessarily be applicable to this product.

NCBI GI #
1102845313
NCBI Accession #
APC94174.1
NCBI Official Full Name
MSH2
UniProt Protein Name
MSH2
UniProt Synonym Protein Names
MSH2
Protein Family
DNA mismatch repair protein
UniProt Gene Name
MSH2

UniProt Comments for MSH2

Component of the post-replicative DNA mismatch repair system (MMR).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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