Rabbit Anti-PD-L1 Monoclonal Antibody – Cat# MBS344033

PD-L1 [IHC411] on Lung

PD-L1 Antibody – Rabbit Monoclonal

Name: PD-L1 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS344033
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS344033

Intended Use: The PD-L1 [IHC411] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test… More details

$280 / 0.1 mL (Concentrate) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PD-L1, Monoclonal Antibody

Full Product Name

PD-L1

Product Gene Name

anti-PD-L1 antibody

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide

Preparation and Storage

Store at 2-8 degree C. Do not freeze.
To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.
Positive and negative controls should be simultaneously run with unknown specimens.

Molecular Weight

20,246 Da

Positive Control

Tonsil, Lung Adenocarcinoma

Principles and Procedures

Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.

Reconstitution

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.

Specimen Collection and Preparation for Analysis

Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).
Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.

Application Notes for anti-PD-L1 antibody

The recommended working dilution range is 1:100 - 1:200.

Product References and Citations for anti-PD-L1 antibody

1. Ostrand-Rosenberg S, et al. J Immunol. 2014; 193:3835-41.
2. Tokito T, et al. Eur J Cancer. 2016; 55:7-14.
3. Park IH, et al. Clin Breast Cancer. 2016; 16:51-8.
4. Kakavand H, et al. Mod Pathol. 2015; 28: 1535-44.
5. Xia B, et al. Immunotherapy. 2016; 8:279-98.
6. Patel SP, et al. Mol Cancer Ther. 2015; 14:847-56.
7. Singh BP, et al. Cancers (Basel). 2016; 8.
8. Chemnitz JM, et al. J Immunol. 2004; 173:945-54.
9. Thompson RH, et al. Proc Natl Acad Sci USA. 2004; 101:17174-9.
10. Hamanishi J, et al. Proc Natl Acad Sci USA. 2007; 104:3360-5.
11. Mozaffarian N, et al. Rheumatology, 2008; 47:1335-41.

Other Notes

Small volumes of anti-PD-L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PD-L1 purchase

MBS344033 is an antibody targeting PD-L1. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PD-L1. It may not necessarily be applicable to this product.

NCBI GI #

930425329

NCBI GeneID

29126

NCBI Accession #

NP_001300958.1

UniProt Secondary Accession #

Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27

UniProt Related Accession #

Q9NZQ7

GenBank Nucleotide #

NM_001314029.1

NCBI Official Full Name

programmed cell death 1 ligand 1 isoform c

NCBI Official Synonym Full Names

CD274 molecule

NCBI Official Symbol

CD274

NCBI Official Synonym Symbols

B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

UniProt Protein Name

Programmed cell death 1 ligand 1

UniProt Synonym Protein Names

B7 homolog 11 PublicationManual assertion based on opinion iniRef.1"B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion."Dong H., Zhu G., Tamada K., Chen L.Nat. Med. 5:1365-1369(1999) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.

UniProt Gene Name

CD274

UniProt Synonym Gene Names

PD-L12 PublicationsManual assertion based on opinion iniRef.10; PDCD1 ligand 1; Programmed death ligand 1; B7-H11 PublicationManual assertion based on opinion iniRef.1

NCBI Protein Information

programmed cell death 1 ligand 1

NCBI Summary for PD-L1

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

UniProt Comments for PD-L1

Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).

Research Articles on PD-L1

1. High CD274 expression is associated with Epithelial Ovarian Cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PD-L1 antibody

Reference Product	PubMed Publications
PDCD1 antibody	>520 publications with PD-L1 and PDCD1
CD80 antibody	>414 publications with PD-L1 and CD80
PDCD1LG2 antibody	>204 publications with PD-L1 and PDCD1LG2
HLA-DRB1 antibody	>1 publications with PD-L1 and HLA-DRB1
LCK antibody	>1 publications with PD-L1 and LCK
PTPN11 antibody	>1 publications with PD-L1 and PTPN11

Pathways associated with anti-PD-L1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Costimulation By The CD28 Family Pathway antibodies	Costimulation By The CD28 Family Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
PD-1 Signaling Pathway antibodies	PD-1 Signaling Pathway Diagram

Diseases associated with anti-PD-L1 antibody

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>185 publications with PD-L1 and Lung Neoplasms
Lung Diseases Antibodies	>171 publications with PD-L1 and Lung Diseases
Inflammation Antibodies	>169 publications with PD-L1 and Inflammation
Adenocarcinoma Antibodies	>140 publications with PD-L1 and Adenocarcinoma
Neoplasms, Experimental Antibodies	>117 publications with PD-L1 and Neoplasms, Experimental
Liver Diseases Antibodies	>112 publications with PD-L1 and Liver Diseases
Necrosis Antibodies	>101 publications with PD-L1 and Necrosis
Nervous System Diseases Antibodies	>99 publications with PD-L1 and Nervous System Diseases
Neoplasm Metastasis Antibodies	>88 publications with PD-L1 and Neoplasm Metastasis
Kidney Diseases Antibodies	>86 publications with PD-L1 and Kidney Diseases

Organs/Tissues associated with anti-PD-L1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1113 publications with PD-L1 and Blood
Lung Antibodies	>240 publications with PD-L1 and Lung
Lymph Node Antibodies	>110 publications with PD-L1 and Lymph Node
Skin Antibodies	>80 publications with PD-L1 and Skin
Brain Antibodies	>51 publications with PD-L1 and Brain
Pancreas Antibodies	>33 publications with PD-L1 and Pancreas
Stomach Antibodies	>28 publications with PD-L1 and Stomach
Placenta Antibodies	>23 publications with PD-L1 and Placenta
Prostate Antibodies	>18 publications with PD-L1 and Prostate
Mouth Antibodies	>15 publications with PD-L1 and Mouth