Mouse Anti-Collagen Type IV Monoclonal Antibody – Cat# MBS344048

Collagen Type IV [IHC549] on Liver

Collagen Type IV Antibody – Mouse Monoclonal

Name: Collagen Type IV Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS344048
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS344048

Intended Use: The Collagen Type IV [IHC549] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test… More details

$230 / 0.1 mL (Concentrate) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Collagen Type IV, Monoclonal Antibody

Full Product Name

Collagen Type IV

Clonality

Monoclonal

Host

Mouse

Species Reactivity

Human

Form/Format

Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide

Preparation and Storage

Store at 2-8 degree C. Do not freeze.
To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.
Positive and negative controls should be simultaneously run with unknown specimens.

Positive Control

Lung, Muscle

Principles and Procedures

Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.

Reconstitution

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.

Specimen Collection and Preparation for Analysis

Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).
Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.

Application Notes for anti-Collagen Type IV antibody

The recommended working dilution range is 1:100 - 1:200.

Product References and Citations for anti-Collagen Type IV antibody

1. Gould VE, et al. Pathol Annul. 1976; 11:353-86.
2. McArdle JP, et al. Int J Cancer. 1984: 34:633-8.
3. Sakr WA, et al. Hum Pathol. 1987; 18:1043-50.
4. Barsky SH, et al. Am J Surg Pathol. 1983; 7:667-77.
5. De Iorio P, et al. Anticancer Res. 2001; 21:1395-9.
6. Maatta M, et al. J Histochem Cytochem. 2001; 49:711-26.
7. Schmehl K, et al. Int J Colorectal Dis. 2000; 15:39-48.
8. Felix A, et al. Hum Pathol. 1999; 30:964-9.
9. Damiani S, et al. Virchows Arch. 1999; 434:227-34.

Other Notes

Small volumes of anti-Collagen Type IV antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Collagen Type IV purchase

MBS344048 is an antibody targeting Collagen Type IV. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Collagen Type IV. It may not necessarily be applicable to this product.

Protein Family

Collagen type IV alpha-3-binding protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.